تأثير برون‌تني سطوح مختلف عصاره نعنافلفلي بر فراسنجه‌هاي كيفي مني در قوچ عربي

In Vitro effect of different levels of peppermint (Mentha piperital) extract on semen quality parameters in Arabi ram

ﻫﺪف از ﺗﺤﻘﯿﻖ ﺣﺎﺿﺮ، ﺑﺮرﺳﯽ ﺗﺄﺛﯿﺮ ﺳﻄﻮح ﻣﺨﺘﻠﻒ ﻋﺼﺎره ﻧﻌﻨﺎع ﻓﻠﻔﻠﯽ ﺑﺮ ﮐﯿﻔﯿﺖ ﻣﻨﯽ ﻗﻮچ ﻋﺮﺑﯽ ﺑﻮد. اﺳﭙﺮم ﮔﯿﺮي از 12 رأس ﻗﻮچ ﻋﺮﺑﯽ ﺑﻪ ﻃﻮر ﻫﻔﺘﮕﯽ ﺑﻪ ﻣﺪت 8 ﻫﻔﺘﻪ اﻧﺠﺎم و ﻣﻨﯽ آﻧﻬﺎ ﺑﻼﻓﺎﺻﻠﻪ ﺑﺎ ﻫﻢ ﻣﺨﻠﻮط ﺷﺪ. ﻧﻤﻮﻧﻪﻫﺎي ﻣﻨﯽ ﻣﺨﻠﻮط و رﻗﯿﻖ ﺷﺪه ﺑﻪ 5 ﻗﺴﻤﺖ ﺗﻘﺴﯿﻢ و ﺳﻄﻮح ﺻﻔﺮ، 150 ،100 ،50 و 200 ﻣﯿﮑﺮوﻟﯿﺘﺮ در ﻣﯿﻠﯽﻟﯿﺘﺮ ﻋﺼﺎره ﻧﻌﻨﺎﻓﻠﻔﻠﯽ درﯾﺎﻓﺖ ﮐﺮدﻧﺪ. در زﻣﺎنﻫﺎي ﻣﺨﺘﻠﻒ ﻧﮕﻬﺪاري ﻣﻨﯽ رﻗﯿﻖ ﺷﺪه ﺣﺎوي ﺗﯿﻤﺎرﻫﺎي آزﻣﺎﯾﺸﯽ )ﺻﻔﺮ، 48 ،24 و 72 ﺳﺎﻋﺖ( ﺑﻪ ﺻﻮرت ﻣﺎﯾﻊ ﺗﺤﺖ دﻣﺎي 5 درﺟﻪ ﺳﻠﺴﯿﻮس، ﻓﺮاﺳﻨﺠﻪﻫﺎي ﮐﯿﻔﯽ ﻣﻨﯽ ﺑﺮرﺳﯽ ﺷﺪﻧﺪ. ﺑﻼﻓﺎﺻﻠﻪ ﭘﺲ از اﺳﭙﺮمﮔﯿﺮي، ﮐﻤﺘﺮﯾﻦ ﻣﯿﺰان ﺗﺤﺮك ﮐﻞ و زﻧﺪهﻣﺎﻧﯽ اﺳﭙﺮم ﻣﺘﻌﻠﻖ ﺑﻪ ﺳﻄﺢ 200 ﻋﺼﺎره ﻧﻌﻨﺎع ﻓﻠﻔﻠﯽ ﺑﻮد )P<0/05(. 24 ﺳﺎﻋﺖ ﭘﺲ از اﺳﭙﺮمﮔﯿﺮي، ﺳﻄﻮح 150 و 200 ﻋﺼﺎره ﻧﻌﻨﺎع ﻓﻠﻔﻠﯽ ﻣﯿﺰان ﻧﺎﻫﻨﺠﺎري ﻫﺎي ﻣﻮرﻓﻮﻟﻮژﯾﮑﯽ اﺳﭙﺮم را اﻓﺰاﯾﺶ دادﻧﺪ. در اﯾﻦ زﻣﺎن، ﮐﻤﺘﺮﯾﻦ ﻣﯿﺰان ﺳﻼﻣﺖ ﻏﺸﺎي ﭘﻼﺳﻤﺎي اﺳﭙﺮم ﻣﺮﺑﻮط ﺑﻪ ﺳﻄﺢ 200 ﻋﺼﺎره ﻧﻌﻨﺎع ﻓﻠﻔﻠﯽ ﺑﻮد )P<0/05(. 48 ﺳﺎﻋﺖ ﭘﺲ از اﺳﭙﺮمﮔﯿﺮي، ﺑﯿﺸﺘﺮﯾﻦ ﺟﻨﺒﺎﯾﯽ ﮐﻞ، زﻧﺪهﻣﺎﻧﯽ و ﺳﻼﻣﺖ ﻏﺸﺎي ﭘﻼﺳﻤﺎﯾﯽ اﺳﭙﺮم ﻣﺮﺑﻮط ﺑﻪ ﮔﺮوه ﺷﺎﻫﺪ ﺑﻮد )P<0/05(. 72 ﺳﺎﻋﺖ ﭘﺲ از اﺳﭙﺮمﮔﯿﺮي، اﺧﺘﻼف آﻣﺎري ﻣﻌﻨﯽداري ﺑﯿﻦ ﺗﯿﻤﺎرﻫﺎ ﺑﺮاي ﺗﻤﺎم ﻓﺮاﺳﻨﺠﻪﻫﺎي ﮐﯿﻔﯽ اﺳﭙﺮم ﯾﺎﻓﺖ ﻧﺸﺪ. در اﯾﻦ زﻣﺎن، ﺑﯿﺸﺘﺮﯾﻦ ﻏﻠﻈﺖ ﻣﺎﻟﻮن ديآﻟﺪﻫﯿﺪ )MDA( ﭘﻼﺳﻤﺎي ﻣﻨﯽ ﻣﺮﺑﻮط ﺑﻪ ﺳﻄﺢ 100 ﻋﺼﺎره ﻧﻌﻨﺎﻓﻠﻔﻠﯽ ﯾﻮد )P<0/05(. ﺑﻪ ﻃﻮرﮐﻠﯽ، ﺑﺎ اﻓﺰودن ﺳﻄﻮح ﻣﺨﺘﻠﻒ ﻋﺼﺎره ﻧﻌﻨﺎﻓﻠﻔﻠﯽ ﺑﻪ رﻗﯿﻖ ﮐﻨﻨﺪه و ﻧﮕﻬﺪاري ﻣﻨﯽ رﻗﯿﻖ ﺷﺪه ﻗﻮچ ﻋﺮﺑﯽ ﺑﻪ ﺣﺎﻟﺖ ﻣﺎﯾﻊ در 5 درﺟﻪ ﺳﻠﺴﯿﻮس، ﻏﻠﻈﺖﻫﺎي ﺑﻪ ﮐﺎر رﻓﺘﻪ ﻋﺼﺎره ﻧﻌﻨﺎعﻓﻠﻔﻠﯽ ﻧﻪ ﺗﻨﻬﺎ ﺳﺒﺐ ﺑﻬﺒﻮد ﻓﺮاﺳﻨﺠﻪﻫﺎي ﮐﯿﻔﯽ اﺳﭙﺮمﻫﺎ ﻧﺸﺪ، ﺑﻠﮑﻪ اﺛﺮ ﻣﺨﺮﺑﯽ ﻫﻢ داﺷﺖ.

The aim of this study was to investigate the effect of different levels of peppermint extract on semen quality of Arabi rams. Semen was collected from 12 Arabi rams weekly for 8 weeks and immediately mixed, and then were divided into 5 parts and received zero, 50, 100, 150 and 200 μl/ml of peppermint extract. At different storage times of diluted semen containing treatments (zero, 24, 48 and 72 hours) in liquid condition, semen quality parameters were evaluated. Immediately after sperm collection, the lowest total motility and sperm viability belonged to the level of 200 peppermint extract (P<0.05). At 24 hours, levels 150 and 200 of extract increased the rate of morphological abnormalities of sperms. At this time, the lowest level of sperm plasma membrane integrity was related to the level of 200 peppermint extracts (P<0.05). 48 hours after sperm storage, the highest total motility, viability and plasma membrane integrity of sperms were belonged to the control group (P<0.05). 72 hours after semen storage, no statistically significant differences were found among treatments for all sperm quality parameters. At this time, the highest concentration of malondialdehyde (MDA) in seminal plasma was related to the level of 100 μl/ml peppermint extract (P<0.05). In general, by adding different levels of peppermint extract to the diluent and maintaining the diluted semen of the Arabi ram in a liquid state at 5°C, the concentrations used of peppermint extract not only did not improve the qualitative parameters of the sperm, it also had a devastating effect.