عنوان مقاله :
اثر چهار هفته ورزش و مكمل‌دهي اولئورپين بر استرس اكسيداتيو بافت مغزي در مدل تجربي بيماري پاركينسون در موش صحرايي
عنوان به زبان ديگر :
The Effect of Four Weeks of Exercise and Oleurpine Supplementation on Oxidative Stress in Brain Tissue in Experimental Model of Parkinson's Disease in Rat
پديد آورندگان :
كشاورزيان، فرشته دانشگاه آزاد اسلامي واحد اهواز، اهواز، ايران , دولاح، عبدالحسن دانشگاه آزاد اسلامي واحد اهواز - گروه زيست‌شناسي، اهواز، ايران , رفيعي راد، مريم دانشگاه آزاد اسلامي واحد اهواز - گروه زيست‌شناسي، اهواز، ايران
كليدواژه :
اﺳﺘﺮس اﮐﺴﯿﺪاﺗﯿﻮ , اوﻟﺌﻮروﭘﯿﻦ , ﭘﺎرﮐﯿﻨﺴﻮن , ﺗﻤﺮﯾﻦ ﺷﻨﺎ , ﻣﻮش ﺻﺤﺮاﯾﯽ
چكيده فارسي :
ﭘﺎرﮐﯿﻨﺴﻮن ﯾﮏ اﺧﺘﻼل ﻋﺼﺒﯽ ﺗﺨﺮﯾﺐ ﮐﻨﻨﺪه ﻣﺰﻣﻦ و ﺷﺎﯾﻊ اﺳﺖ. در اﯾﻦ ﻣﻄﺎﻟﻌﻪ اﺛﺮات ﺗﻤﺮﯾﻦ ﺷﻨﺎ و ﻣﮑﻤﻞ دﻫﯽ اوﻟﺌﻮرﭘﯿﻦ ﺑﺮ اﺳﺘﺮس اﮐﺴﯿﺪاﺗﯿﻮ ﺑﺎﻓﺖ ﻣﻐﺰي در ﻣﺪل ﺗﺠﺮﺑﯽ ﺑﯿﻤﺎري ﭘﺎرﮐﯿﻨﺴﻮن ﺑﺮرﺳﯽ ﮔﺮدﯾﺪ. در اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺗﺠﺮﺑﯽ، 40 ﺳﺮ ﻣﻮش ﺻﺤﺮاﯾﯽ ﻧﺮ ﺑﺎﻟﻎ ﻧﮋاد وﯾﺴﺘﺎر، اﻧﺘﺨﺎب و ﺑﻪ ﭘﻨﺞ ﮔﺮوه ﺷﺎﻣﻞ ﮔﺮوه ﮐﻨﺘﺮل، ﮔﺮوه ﭘﺎرﮐﯿﻨﺴﻮن، ﺗﻤﺮﯾﻦ ﺷﻨﺎ، ﻣﺼﺮف اوﻟﺌﻮروﭘﯿﻦ، ﺗﻤﺮﯾﻦ ﺷﻨﺎ ﻫﻤﺮاه ﺑﺎ ﻣﺼﺮف اوﻟﺌﻮروﭘﯿﻦ ﺗﻘﺴﯿﻢ ﺷﺪﻧﺪ. ﮔﺮوه ﻫﺎ ﺑﻪ ﻣﺪت ﭼﻬﺎر ﻫﻔﺘﻪ )ﭘﻨﺞ ﺟﻠﺴﻪ در ﻫﻔﺘﻪ و ﻫﺮ ﺟﻠﺴﻪ 30 دﻗﯿﻘﻪ( ﺷﻨﺎي اﺟﺒﺎري ﮐﺮدﻧﺪ و ﮔﺮوه ﻫﺎي 4 و 5 ﺑﻪ ﻣﺪت ﭼﻬﺎر ﻫﻔﺘﻪ، روزاﻧﻪ اوﻟﺌﻮروﭘﯿﻦ را ﺑﻪ ﻣﯿﺰان 20 ﻣﯿﻠﯽ ﮔﺮم ﺑﻪ ازاي ﻫﺮ ﮐﯿﻠﻮﮔﺮم وزن ﺑﺪن، ﺑﻪ ﺻﻮرت اورال درﯾﺎﻓﺖ ﮐﺮدﻧﺪ. ﺳﻄﻮح ﺳﺮﻣﯽ ﻣﺘﻐﯿﺮﻫﺎ ﺑﺎ اﺳﺘﻔﺎده از روش ﺗﯿﻮﺑﺎرﺑﯿﺘﻮرﯾﮏ اﺳﯿﺪ ﻣﻮرد ارزﯾﺎﺑﯽ ﻗﺮار ﮔﺮﻓﺖ. داده ﻫﺎ ﺑﺎ اﺳﺘﻔﺎده ازآزﻣﻮن ﻫﺎي آﻣﺎري وارﯾﺎﻧﺲ ﯾﮏ ﻃﺮﻓﻪ و ﺗﻌﻘﯿﺒﯽ ﺗﻮﮐﯽ ﺗﺠﺰﯾﻪ و ﺗﺤﻠﯿﻞ ﺷﺪﻧﺪ )0/05≤p(. در اﯾﻦ ﻣﻄﺎﻟﻌﻪ، اوﻟﺌﻮروﭘﯿﻦ- ﭘﺎرﮐﯿﻨﺴﻮن، ﺗﻤﺮﯾﻦ ﺷﻨﺎ- ﭘﺎرﮐﯿﻨﺴﻮن و ﺗﻤﺮﯾﻦ ﺷﻨﺎ ﻫﻤﺮاه ﺑﺎ ﻣﺼﺮف اوﻟﺌﻮروﭘﯿﻦ- ﭘﺎرﮐﯿﻨﺴﻮن، ﮐﺎﻫﺶ ﻣﻌﻨﯽ داري ﺑﺮ ﺳﻄﺢ ﻣﺎﻟﻮن دي آﻟﺪﻫﯿﺪ، اﻓﺰاﯾﺶ ﻣﻌﻨﯽ داري ﺑﺮ ﻣﯿﺰان ﻓﻌﺎﻟﯿﺖ ﮔﻠﻮﺗﺎﺗﯿﻮن ﭘﺮاﮐﺴﯿﺪاز و اﻓﺰاﯾﺶ ﻣﻌﻨﯽ داري ﺑﺮ ﻣﯿﺰان ﺗﯿﻮل در ﻣﻘﺎﯾﺴﻪ ﺑﺎ ﮔﺮوه ﭘﺎرﮐﯿﻨﺴﻮﻧﯽ داﺷﺘﻨﺪ )P<0/001(. ﺗﻤﺮﯾﻦ ﺷﻨﺎ ﻫﻤﺮاه ﺑﺎ ﻣﺼﺮف اوﻟﺌﻮروﭘﯿﻦ ﻣﯽ ﺗﻮاﻧﺪ دﻓﺎع آﻧﺘﯽ اﮐﺴﯿﺪاﻧﯽ را ﺑﻬﺒﻮد ﺑﺨﺸﯿﺪه و ﭘﺮاﮐﺴﯿﺪاﺳﯿﻮن ﻟﯿﭙﯿﺪي را ﮐﺎﻫﺶ دﻫﺪ. ﺑﻨﺎﺑﺮاﯾﻦ، اﺣﺘﻤﺎﻻً اﯾﻦ اﺛﺮ ﺗﻌﺎﻣﻠﯽ ﺑﺘﻮاﻧﺪ ﺷﯿﻮه درﻣﺎﻧﯽ ﻣﺆﺛﺮ و ﭘﯿﺸﮕﯿﺮاﻧﻪ اي ﺑﺮاي ﺟﻠﻮﮔﯿﺮي از ﺑﯿﻤﺎري ﻫﺎي ﻣﺮﺗﺒﻂ ﺑﺎ اﺳﺘﺮس اﮐﺴﺎﯾﺸﯽ ﺑﺎﺷﺪ.
چكيده لاتين :
Parkinson is a chronic and widespread
neurodegenerative disorder. In this study, the effects
of swimming exercise and oleuropein
supplementation on oxidative stress of brain tissue in
experimental model of Parkinson's disease in rat were
investigated. In this experimental study, 40 adult
male Wistar rats were selected and divided into five
groups including: control group, Parkinson's group,
swimming exercise, oleuropein consumption,
swimming exercise with oleuropein consumption.
The groups were forced to swim for 4 weeks (five
sessions per week for 30 minutes each session) and
groups 4 and 5 received 20 mg of oleuropein daily
per kilogram of body weight orally for 4 weeks.
Serum levels of the variables were evaluated using
the thiobarbituric acid method. Data were analyzed
using one-way ANOVA and Tukey post hoc tests
(p≥0.05). In this study, oleuropein-Parkinson,
swimming exercise-Parkinson, and swimming
exercise with oleuropein-Parkinson consumption
significantly decreased Malondialdehyde levels,
significantly increased glutathione peroxidase activity
and thiol compared to Parkinson's group (P<0/001).
Swimming exercise with oleuropein consumption can
improve antioxidant defense and decrease lipid
peroxidation. Therefore, this interactive effect may
necessitate more therapeutic and preventive treatment
to prevent oxidative stress-related diseases
عنوان نشريه :
زيست شناسي جانوري تجربي
