بهينه‌سازي توليد رنگدانه كانتاگزانتين توسط سويه مقاوم به اشعه ديتزيا ماريس و ارزيابي اثرات آن بر كشت سلولي

Production optimization of canthaxanthin pigment from radioresistant Dietzia maris and evaluation of its effect on cell culture

ﮐﺎروﺗﻨﻮﺋﯿﺪﻫﺎ آﻧﺘﯽاﮐﺴﯿﺪانﻫﺎي زﯾﺴﺘﯽ ﺑﻮده و در ﺣﻔﺎﻇﺖ ﺑﺪن در ﻣﻘﺎﺑـﻞ ﺑﯿﻤـﺎري ﻫـﺎ و ﭘﺪﯾﺪه ﭘﯿﺮي ﻧﻘﺶ ﻣﻬﻤﯽ اﯾﻔﺎ ﻣﯽﮐﻨﻨﺪ. ﮐﺎﻧﺘـ ﺎﮔﺰاﻧﺘﯿﻦ ﯾﮑـﯽ از ﭘﺮﮐـﺎرﺑﺮدﺗﺮﯾﻦ ﮐﺎروﺗﻨﻮﺋﯿـﺪﻫﺎ در ﺻـﻨﻌﺖ و ﭘﺰﺷﮑﯽ ﻣﯽﺑﺎﺷﺪ. ﻫﺪف از اﯾـﻦ ﭘـﮋوﻫﺶ، ﻣﻄﺎﻟﻌـﻪ ﺧـﻮاص زﯾﺴـﺘﯽ رﻧﮕﺪاﻧـﻪ ﮐﺎﻧﺘـﺎﮔﺰاﻧﺘ ﯿﻦ و ﻫﻤﭽﻨـﯿﻦ ﺑﻬﯿﻨﻪﺳﺎزي ﺗﻮﻟﯿﺪ آن در ﻣﺤﯿﻂ ﮐﺸﺖ ارزان ﻗﯿﻤﺖ ﺗﻮﺳﻂ ﺳﻮﯾﻪ ﻣﯿﮑﺮوﺑﯽ ﻣﻘﺎوم ﺑﻪ اﺷﻌﻪ دﯾﺘﺰﯾـﺎ ﻣـﺎرﯾﺲ ﺑﻮده اﺳﺖ. ﻣﻮاد و روشﻫﺎ: ﮐﺎروﺗﻨﻮﺋﯿﺪ ﺑﺎﮐﺘﺮﯾﺎﯾﯽ، اﺳﺘﺨﺮاج و ﺧـﻮاص ﺿـﺪ ﺑﺎﮐﺘﺮ ﯾـﺎﯾﯽ، ﺿـﺪ ﺗﻮﻣـﻮر ي و ﺳـﻤﯿﺖ ﺳﻠﻮﻟﯽ آن ﺑﺮرﺳﯽ ﺷﺪ. ﺳﭙﺲ ﺑﺎ اﺳﺘﻔﺎده از روش ﺷﻨﺎﺳﯽ ﺳﻄﺢ ﭘﺎﺳﺦ اﺛﺮ واﺳﻄﻪﻫـﺎي ﭼﺮﺧـﻪ ﮐـﺮﺑﺲ و pH، ﺑﺮ ﺗﻮﻟﯿﺪ رﻧﮕﺪاﻧﻪ و زﯾﺴﺖ ﺗﻮده ﻣﯿﮑﺮوﺑﯽ در ﻣﺤﯿﻂ ﮐﺸﺖ آب ﭘﻨﯿﺮ ﻣﻮرد ﺑﺮرﺳﯽ ﻗﺮار ﮔﺮﻓﺖ. ﯾﺎﻓﺘﻪﻫﺎ: ﺣﺪاﮐﺜﺮ ﺗﻮﻟﯿﺪ رﻧﮕﺪاﻧﻪ ﺑﻪ ﻣﯿﺰان92/54 ﻣﯿﻠﯽﮔﺮم ﺑﺮ ﻟﯿﺘﺮ در ﻣﺤﯿﻂ ﮐﺸﺖ آب ﭘﻨﯿﺮ در 8 pH و در ﺣﻀﻮر 12/5 mM از ﺳﯿﺘﺮات، ﮔﻠﻮﺗﺎﻣﺎت، ﻣﺎﻻت، و ﺳﻮﮐﺴﯿﻨﺎت ﺗﻮﺳﻂ روش ﺳﻄﺢ ﭘﺎﺳﺦ ﺑﻪ دﺳﺖ آﻣﺪ. رﻧﮕﺪاﻧﻪ ﻓﻮق ﻫﯿﭽﮕﻮﻧﻪ اﺛﺮ ﺳﻤﯿﺖ ﺳﻠﻮﻟﯽ ﺳﻤﯿﺘﯽ ﺑﺮ ردهﻫﺎي ﺳﻠﻮﻟﯽHFB ،Hela وMCF-7 ﻧﺸﺎن ﻧﺪاد. ﺑﻪ ﻋﻼوه، رﻧﮕﺪاﻧﻪ ﻓﺎﻗﺪ ﺧﺎﺻﯿﺖ ﺿﺪ ﺑﺎﮐﺘﺮﯾﺎﯾﯽ ﺑﻮد. ﺑﺤﺚ: ﺳﻮﯾﻪﻫﺎي ﻣﯿﮑﺮوﺑﯽ ﻣﻘﺎوم ﺑﻪ اﺷﻌﻪ، ﺑﻪ دﻟﯿﻞ ﭘﺎﯾﺪاري آﻧﻬﺎ و ﻓﻌﺎﻟﯿـ ﺖ آﻧﺘـﯽ اﮐﺴـﯿﺪاﻧﯽ ﺑـﺎﻻ ﮐﺎﻧﺪﯾـ ﺪ ﺑﻬﺘﺮي در ﺗﻮﻟﯿﺪ رﻧﮕﺪاﻧﻪﻫﺎي ﻣﯿﮑﺮوﺑﯽ ﻣﺤﺴﻮب ﻣﯽﺷﻮﻧﺪ. در اﯾﻦ ﻣﻄﺎﻟﻌﻪ، در ﺟﻬﺖ ﮐﺎﻫﺶ ﻫﺰﯾﻨﻪ ﺗﻮﻟﯿـﺪ ﮐﺎﻧﺘﺎﮔﺰاﻧﺘﯿﻦ از ﻣﺤﯿﻂ ﮐﺸﺖ آب ﭘﻨﯿﺮ اﺳﺘﻔﺎده ﺷﺪ. اﻓﺰودن واﺳﻄﻪﻫﺎي ﮐﺮﺑﺲ در ﻣﺤـ ﯿﻂ ﮐﺸـﺖ ﺗﺨﻤ ﯿـ ﺮ،ﺗﻮﻟﯿﺪ رﻧﮕﺪاﻧﻪ ﺗﻮﺳﻂ دﯾﺘﺰﯾﺎ ﻣﺎرﯾﺲﺑﻪ ﻃﻮر ﭼﺸﻤﮕﯿﺮي اﻓﺰاﯾﺶ داد.

Carotenoids are biological antioxidants and play important roles in protecting the body from diseases and aging. Canthaxanthin is one of the most widely used carotenoids in the industry and medicine. This study aimed to investigate the biological properties of canthaxanthin pigment as well as its production optimization in a low-cost medium using a radioresistant microbial strain named Dietzia maris. Materials and methods: Bacterial carotenoids were extracted and its antibacterial, anti-tumor, and cytotoxicity properties were investigated. Then, the effect of Krebs intermediates and pH on the production of pigment and microbial biomass in the whey medium was investigated using the response surface methodology. Results: Maximum pigment production was found to be 92/54 mg/l in whey culture medium at pH 8 and in the presence of 12.5 mM of each of citrate, glutamate, malate, and succinate by the response surface method. The pigment did not show any cytotoxic effect on Hela, HFB, and MCF-7 cell lines. Besides, the pigment did not have any antibacterial properties. Conclusion: Radioresistant microbial strains are better candidates for microbial pigment production due to their stability and high antioxidant activity. In this study, a whey culture medium was used to reduce the production cost of canthaxanthin. The addition of Krebs intermediaries in the fermentation medium increased the pigment production by Dietzia maris significantly.