ﺳﻨﺘﺰ زﯾﺴﺘﯽ ﻧﺎﻧﻮ ذرات ﻧﻘﺮه ﺑﺎ اﺳﺘﻔﺎده از ﻋﺼﺎره آﺑﯽ ﻣﯿﻮه ﮔﻮاوا (Psidium guajava) و ﺑﺮرﺳﯽ ﻓﻌﺎﻟﯿﺖ آﻧﺘﯽ ﺑﺎﮐﺘﺮﯾﺎل آن

Biological synthesis of silver nanoparticles using the aqueous extract of Psidium guajava (PG) and its antibacterial activity

در ﻣﺠﻤﻮﻋﻪ ﮐﺎرﺗﺤﻘﯿﻘﺎﺗﯽ، ﻋﺼﺎره ﮔﯿﺎﻫﯽ ﻣﯿﻮه ﮔﻮاوا1 ﺑﻪ دﻟﯿﻞ دارا ﺑﻮدن ﺧﺎﺻﯿﺖ ﮐﺎﻫﻨﺪﮔﯽ، ﺑﺮاي ﺳـﻨﺘﺰ ﻧﺎﻧﻮ ذرات ﻧﻘﺮه )Ag-NPs( ﻣﻮرد ﺑﺮرﺳﯽ ﻗﺮار ﮔﺮﻓﺖ. ﺑﺎ ﺗﻮﺟﻪ ﺑﻪ آﺛﺎر ﮐﺎﻫﻨﺪﮔﯽ و ﻣﺤﺎﻓﻈﺖ ﺧﻮب ﮔﻮاوا )PG (، ﺳﻨﺘﺰ ﻧﺎﻧﻮذرات ﻧﻘﺮه را در اﺑﻌﺎد ﻧﺎﻧﻮ ﻣﻬﯿﺎ ﻣﯽﮐﻨﺪ. ﻃﯿﻒ ﺳﻨﺠﯽ اﺷﻌﻪ ﻣﺎوراﺑﻨﻔﺶ )UV-vis(، ﭘﺮاش-اﺷﻌﻪاﯾﮑﺲ )XRD(، ﻃﯿﻒ ﺳﻨﺠﯽ ﻣﺎدون ﻗﺮﻣﺰ ﺗﺒﺪﯾﻞ ﻓﻮرﯾﻪ )FTIR(، ﻣﯿﮑﺮوﺳـﮑﻮپ اﻟﮑﺘﺮوﻧـ ﯽ ﻋﺒـﻮري )TEM(، ﻣﯿﮑﺮوﺳﮑﻮپ اﻟﮑﺘﺮوﻧﯽ روﺑﺸﯽ )SEM( و ﻃﯿﻒ ﺳﻨﺠﯽ اﻧﺮژي اﺷـﻌﻪ اﯾﮑـﺲ )EDAX( ﺑـﺮاي ﺷﻨﺎﺳﺎﯾﯽ ﻧﺎﻧﻮ ذرات ﻧﻘﺮه ﺳﻨﺘﺰ ﺷﺪه ﺑﺎ اﺳﺘﻔﺎده از روش زﯾﺴﺘﯽ ﺑﻪﮐـﺎر ﺑـﺮده ﺷـﺪه اﺳـﺖ . دادهﻫـﺎ ﻧﺸـﺎن ﻣﯽدﻫﻨﺪ ﮐﻪ در اﺛﺮ ﺳﻨﺘﺰ زﯾﺴﺘﯽ، ﻧﺎﻧﻮ ذرات ﻧﻘﺮه ﺑﺎ ﻗﻄﺮﻫﺎي10-20 ﻧﺎﻧﻮﻣﺘﺮ ﺗﻮﻟﯿـ ﺪ ﻣـ ﯽﺷـﻮ ﻧﺪ. ﻣﻄﺎﻟﻌـﺎت XRD درﺟﻪ ﺑﺎﻻﯾﯽ از ﺑﻠﻮرﻫﺎي ﻧﺎﻧﻮذرات ﻧﻘﺮه، ﻣﻨﺤﺼﺮ ﺑﻪ ﻓﺮد ﺑﺎ ﺳﺎﺧﺘﺎر ﻣﮑﻌﺒﯽ ﻣﺮﮐﺰ ﭘﺮ )fcc( را ﻧﺸﺎن ﻣﯽدﻫﺪ. ﻧﺘﺎﯾﺞFTIR ﺑﻪ وﺿﻮح ﻧﺸﺎن داد ﮐﻪ ﻋﺼﺎره ﮔﻮاوا )PG (، ﺣﺎوي-OH ﺑﻪﻋﻨﻮان ﮔﺮوه ﻋـﺎﻣﻠﯽ در واﮐﻨﺶ ﮐﺎﻫﺶ و ﺳﻨﺘﺰ ﻧﺎﻧﻮذرات ﻧﻘﺮه ﻋﻤﻞ ﻣﯽﮐﻨﻨﺪ. ﻓﻌﺎﻟﯿﺖ ﺿﺪ ﺑـﺎﮐﺘﺮ يAg-NPs در ﺑﺮاﺑـﺮ رﺷـﺪ ﮔـﺮ م ﻣﺜﺒﺖ )S. aureus( ﺑﺎ اﺳﺘﻔﺎده از SEM ﻣﻮرد آزﻣﺎﯾﺶ ﻗﺮار ﮔﺮﻓﺖ. ﻣﻬﺎر در رﺷﺪ ﮔﺮم ﻣﺜﺒﺖ )S. aureus( در ﺑﺮاﺑﺮ Ag-NPs ﻣﺸﺎﻫﺪه ﺷﺪ. ﻧﺘﺎﯾﺞ ﻧﺸﺎن ﻣﯽدﻫﺪ ﮐﻪ ذرات ﻧﻘﺮه ﺳﻨﺘﺰ ﺷﺪه ﺑﻪ ﻋﻨﻮان ﻋﺎﻣﻞ ﺿﺪ ﺑﺎﮐﺘﺮي ﻣﺆﺛﺮ ﻋﻤﻞ ﻣﯽ ﮐﻨﺪ. اﯾﻦ ﺛﺎﺑﺖ ﺷﺪه اﺳﺖ ﮐﻪ ذرات ﻧﻘﺮه ﺳﻨﺘﺰ ﺷﺪه ﻗﺎدر ﺑﻪ اراﺋﻪ آﺛﺎر ﺿﺪ ﺑﺎﮐﺘﺮي ﺑﺎﻻﺳـﺖ و از اﯾﻦ رو ﭘﺘﺎﻧﺴﯿﻞ ﺑﺎﻻﯾﯽ در آﻣﺎدهﺳﺎزي داروﻫﺎي ﻣﻮرد اﺳﺘﻔﺎده در ﺑﺮاﺑﺮ ﺑﯿﻤﺎري ﻫﺎ دارد.

In the present work, we describe the synthesis of silver nanoparticles (Ag- NPs) using seed aqueous extract of Psidium guajava (PG) and its antibacterial activity. UV–visible spectroscopy, X-ray diffraction (XRD), Fourier transform infra red spectroscopy (FTIR), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and X-ray energy dispersive spectrophotometer (EDAX) were performed to ascertain the formation of Ag-NPs. It was observed that the growths of Ag-NPs are stopped within 35 min of reaction time. The synthesized Ag-NPs were characterized by a peak at 446 nm in the UV–visible spectrum. XRD confirmed the crystalline nature of the nanoparticles of 10-20 nm size. The XRD peaks at 38◦, 44◦, 64◦ and 77◦ can be indexed to the (1 1 1), (2 0 0), (2 2 0) and (3 1 1) Bragg’s reflections of cubic structure of metallic silver, respectively. The FTIR result clearly showed that the extracts containing OH as a functional group act in capping the nanoparticles synthesis. Antibacterial activities of Ag-NPs were tested against the growth of Grampositive (S. aureus) using SEM. The inhibition was observed in the Ag-NPs against S. aureus. The results suggest that the synthesized Ag-NPs act as an effective antibacterial agent. It is confirmed that Ag-NPs are capable of rendering high antibacterial efficacy and hence has a great potential in the preparation of used drugs against bacterial diseases. The results confirmed that the (PG) is a very good eco friendly and nontoxic source for the synthesis of Ag-NPs as compared to the conventional chemical/physical methods.