تأثير استفاده مجزا و تلفيقي پروبيوتيك Pediococcus acidilactici و پربيوتيك Raffinos بر برخي شاخص‌هاي خوني و شاخص‌هاي ايمني غيراختصاصي سرم در ماهي كاراس طلايي (Carassius auratus)

The effects of singular or combined administration of Pediococcus acidilactici and Raffinos on blood and non-specific immune parameters of goldfish (Carassius auratus)

بررسي شاخص هاي خوني و ايمني يكي از موارد بسيار مهم در زمينه بررسي سلامت آبزيان است و تغذيه نقش مهمي در حفظ شرايط بهداشتي آبزيان با تنظيم سيستم ايمني غيرسلولي و هومورال دارد. اين تحقيق به منظور بررسي اثر استفاده مجزا و تلفيقي پروبيوتيك Pediococcus acidilactici و پربيوتيك Raffinos در جيره غذايي بر شاخص‌هاي خوني و ايمني غيراختصاصي سرم در ماهي طلايي (Carassius auratus) انجام گرفت. تعداد 180 قطعه ماهي طلايي با ميانگين وزني 3/18±26/0 گرم، پس از 10 روز دوره سازگاري با شرايط آزمايشگاه در 4 تيمار با 3 تكرار به مدت 2 ماه تغذيه شدند. تيمارهاي آزمايش شامل: جيره تجاري (گروه شاهد) و به ترتيب غذاي تجاري مكمل شده به P. acidilactici (0/9 گرم بركيلوگرم)، Raffinos (10گرم بركيلوگرم) و غذاي تجاري حاوي سين بيوتيك P. acidilactici و Raffinos بودند. طبق نتايج در شاخص هاي خوني هيچ‌گونه اختلاف معني داري بين تيمارها نسبت به گروه شاهد ديده نشد (0/05

Examination of blood and immune indicators is one of the most important items in the field of aquatic health and nutrition plays an important role in maintaining aquatic health conditions by regulating the non-cellular and humoral immune systems. This study was performed to investigate the effect of singular or combined administration of Pediococcus acidilactici and Raffinos on blood indices and non-specific immune parameters in the diet of goldfish (Carassius auratus). 180 goldfish with an average weight of 26.0±3.18, after 10 days of adaptation to laboratory conditions, were fed in 4 treatments with 3 repetitions for 2 months. Experimental treatments included: commercial diet (control group), commercial diet supplemented with 0.9 g kg-1 P. acidilactici, 10 g kg-1 Raffinos and simultaneously supplemented with P. acidilactici and Raffinos. According to the results, there was no significant difference between the treatments in the blood indicators compared to the control group (P>0.05). The comparison was with the control group, however, there was no significant difference between the control group and the treatments (P>0.05). At the end of the study, The results revealed no significant differences with this dose amongst blood indices and non-specific immune parameters in any of the experimental groups(P>0.05). Therefore, it is necessary to use the right dose of the desired adjuvant, because if irrelevant doses are used, no changes in the aquatic immune system may occur. Excessive doses suppress the immune response, and lower doses do not affect pathogens.