اﺛﺮ ﺗﻤﺮﯾﻦ ﺗﻨﺎوﺑﯽ ﺷﺪﯾﺪ و ﺗﺪاوﻣﯽ ﻣﺘﻮﺳﻂ ﺑﺮ رﮔﺰاﯾﯽ در رﺗﻬﺎي ﻣﺒﺘﻼ ﺑﻪ اﻧﻔﺎرﮐﺘﻮس ﻣﯿﻮﮐﺎرد

The Effects of High Intensity Interval Training and Moderate Continue Training Exercise Training Programs on Angiogeneisi in Rats with Myocardial Infarction

اﻧﻔﺎرﮐﺘﻮس ﻣﯿﻮﮐﺎرد ﯾﮑﯽ از ﻣﻬﻤﺘﺮﯾﻦ ﻋﻠﻞ ﻣﺮگ و ﻣﯿﺮ در ﺟﻬﺎن اﺳﺖ. ﻫﺪف اﯾﻦ ﭘﮋوﻫﺶ ﺑﺮرﺳﯽ ﺗﺄﺛﯿﺮ دو ﻧﻮع ﺑﺮﻧﺎﻣﻪ ﺗﻤﺮﯾﻨﯽ ﺗﻨﺎوﺑﯽ ﺷﺪﯾﺪ و ﺗﺪاوﻣﯽ ﻣﺘﻮﺳﻂ ﺑﺮ ﺑﺮﺧﯽ ﻋﻮاﻣﻞ آﻧﮋﯾﻮژﻧﯿﮏ در رتﻫﺎ ﺑﻌﺪ از اﻟﻘﺎي اﻧﻔﺎرﮐﺘﻮس ﻣﯿﻮﮐﺎرد ﺗﺠﺮﺑﯽ اﺳﺖ. ﻣﻮاد و روشﻫﺎ: 40 ﺳﺮ رت ﻧﺮ وﯾﺴﺘﺎر 12-10 ﻫﻔﺘﻪ ﺑﺎ ﻣﯿﺎﻧﮕﯿﻦ وزﻧﯽ 300-250 ﮔﺮم ﺧﺮﯾﺪاري ﺷﺪﻧﺪ. ﺳﭙﺲ ﺗﺤﺖ ﻋﻤﻞ ﺟﺮاﺣﯽ ﺑﺮاي اﻧﺴﺪاد ﺳﺮﺧﺮگ ﮐﺮوﻧﺮي ﭼﭗ )LAD( ﻗﺮارﮔﺮﻓﺘﻨﺪ. ﺑﺮاي ارزﯾﺎﺑﯽ MI از اﮐﻮﮐﺎردﯾﻮﮔﺮاﻓﯽ اﺳﺘﻔﺎده ﺷﺪ. رتﻫﺎ ﺑﻪ ﭼﻬﺎر ﮔﺮوه، ﮐﻨﺘﺮل )ﺑﺪون ﺗﻤﺮﯾﻦ(، ﺷﻢ و دو ﮔﺮوه ﺗﻤﺮﯾﻨﯽ ﺑﺎ 42 دﻗﯿﻘﻪ دوﯾﺪن روي ﺗﺮدﻣﯿﻞ 8 ﻫﻔﺘﻪ و 5 روز در ﻫﻔﺘﻪ، ﺗﻤﺮﯾﻦ ﺗﻨﺎوﺑﯽ ﺷﺪﯾﺪ )4 دﻗﯿﻘﻪ ﺑﺎ 90-85 و 3 دﻗﯿﻘﻪ ﺑﺎ 50-40 درﺻﺪ VO2max (، ﺗﻤﺮﯾﻦ ﺗﺪاوﻣﯽ ﻣﺘﻮﺳﻂ )ﺑﺎ ﺷﺪت 65-60% VO2max (، ﺗﻘﺴﯿﻢ ﺷﺪﻧﺪ. 48 ﺳﺎﻋﺖ ﭘﺲ از آﺧﺮﯾﻦ ﺟﻠﺴﻪ ﺗﻤﺮﯾﻨﯽ رتﻫﺎ ﺑﯿﻬﻮش و ﻗﻠﺐ آنﻫﺎ ﺗﺤﺖ ﺷﺮاﯾﻂ اﺳﺘﺮﯾﻞ ﺟﺪا ﺷﺪ. ﺑﺮاي ارزﯾﺎﺑﯽ ﺑﯿﺎن ﭘﺮوﺗﺌﯿﻦﻫﺎي eNOS, VEGF, CD34 از روش اﯾﻤﻨﻮﻫﯿﺴﺘﻮﺷﯿﻤﯽ و ﺑﺮاي ارزﯾﺎﺑﯽ ﻋﺎﻣﻞ ﻓﻮن وﯾﻠﺒﺮاﻧﺪ )vWF ( از روش وﺳﺘﺮن ﺑﻼت اﺳﺘﻔﺎده ﺷﺪ. ﺑﻌﺪ از ﺟﻤﻊآوري دادهﻫﺎ، ﺑﺮاي ﺗﺠﺰﯾﻪ و ﺗﺤﻠﯿﻞ اﻃﻼﻋﺎت از آزﻣﻮن ﺗﺤﻠﯿﻞ وارﯾﺎﻧﺲ ﯾﮏ ﺳﻮﯾﻪ و آزﻣﻮن ﺗﻌﻘﯿﺒﯽ ﺗﻮﮐﯽ در ﺳﻄﺢ ﻣﻌﻨﯽداري 0/05

Myocardial Infarction is one of greatest cause of death in the worldwide. Exercise and Physical activity can reduce the myocardail infarction induced phatologic hyperthrophy and dysfunction, and it is a treatment intervention after MI. The mechanism of exercise-induced benefits and angiogenesis in ischemic heart disease remains poorly defined. This study was designed to compare the effects of eight weeks of high intensity interval training and moderate intensity continious training on telocytes and some of angiogenic factors in rats with myocardial infarction. Methods: 40 male Wistar rats weighing 250 to 300 grams were assigned into four groups: Two experimental group run 42 minutes on a treadmill, five days per week for eight weeks; HIIT (each interval four minutes with intensity of 85-90 and three minutes of active recovery with 40% VO2max) and MICT (runing with intensity 60-65% V02max ), the control group (without training intervention) and Sham (surgery control). The expression of protein expression of vWF was investigated by Western Blotting test. To investigate protein expression of CD34, VEGF and eNOS used immunohistochemical tecnique. Data were analyzed using SPSS (version 26) with one way ANOVA (p ≤ 0.05). Results: The results of one-way ANOVA showed that there were significant differences between the four groups of sham, control, HIIT and MICT in Vimentin index (P < 0.0001), c-Kit (P < 0.0001), PDGFRβ (P < 0.0001) and CD34 (P < 0.0001). The markers of thelocyte are more expressed in HIIT than MICT and control groups. The expression of protein of VEGF index (F = 97.05, P <0.0001), eNOS (F = 138.9, P < 0.0001) was significantly different between the groups. Angiogenic factors increased HIIT and MICT, but the difference between the two groups was not significant. vWF index also increased as a result of HIIT, vWF(P <0.005). Conclusion: It is concluded that both HIIT and MICT can help improve myocardial infarction by increasing angiogenesis and blood flow, but HIIT seems to be more effective in this regard.