طراحي، ساخت و استانداردسازي داروي گياهي تقويت كننده حافظه مبتني بر طب سنتي ايران

Design, reformulation, and standardization of a traditional-based memory enhancer herbal preparation originated from Persian medicine

آلزايمر، شايع ترين نوع دمانس مي باشد كه در طب سنتي تحت عناويني همچون "فراموشكاري"، "فساد ذكر" و "نسيان" ذكر شده است. با توجه به چالش هاي اين بيماري، تحقيقات جهت دستيابي به تركيبات طبيعي موثر در آن حايز اهميت مي باشد. هدف هدف، ارايه ي قرص گياهي استاندارد موثر در پيشگيري و درمان آلزايمر مبتني بر طب سنتي ايراني مي باشد. روش بررسي فرآورده هاي مركبه مرتبط از كتب مرجع طب سنتي ايران گردآوري شد. از ميان 127 فرمولاسيون، فرآورده حاوي زيره ايراني و فلفل سياه از كتاب قانون ابن سينا انتخاب و پس از ارزيابي اكسپيان هاي مختلف، فرمولاسيون حاوي 40 درصد زيره ايراني، 40 درصد فلفل سياه و 20 درصد HPMC و PVP به عنوان محلول چسباننده، تهيه گرديد. فرمولاسيون نهايي و هريك از اجزاي آن جهت ارزيابي GC ﻣﻨﻈﻮر ﺗﻬﯿﻪ اﺛﺮ اﻧﮕﺸﺖ HPTLC و ﺗﻌﯿﯿﻦ ﻣﯿﺰان آﻟﮑﺎﻟﻮﺋﯿﺪ ﺗﺎم ﻣﻮﺟﻮد در ﻗﺮص ﺑﺎ اﺳﺘﻔﺎده از روش ﻃﯿﻒﺳﻨﺠﯽ اﻧﺠﺎم ﺷﺪ. ﻧﺘﺎﯾﺞ: آﻧﺎﻟﯿﺰ اﺳﺎﻧﺲ ﻗﺮص ﻣﻮﯾﺪ ﺣﻀﻮر دو ﺗﺮﮐﯿﺐ ﺗﺮاﻧﺲ ﮐﺎرﯾﻮﻓﯿﻠﻦ )29/22 درﺻﺪ( و ﮔﺎﻣﺎﺗﺮﭘﯿﻨﻦ )17/72 درﺻﺪ( ﺑﻮد و اﺳﺘﺎﻧﺪاردﺳﺎزي ﻗﺮص ﺑﺮاﺳﺎس اﯾﻦ دو ﺗﺮﮐﯿﺐ اﻧﺠﺎم ﺷﺪ؛ ﺑﻪ ﮔﻮﻧﻪاي ﮐﻪ ﺑﺼﻮرت ﻣﯿﺎﻧﮕﯿﻦ در ﻫﺮ ﻗﺮص 0/041 ﻣﯿﻠﯽﮔﺮم ﺗﺮاﻧﺲ ﮐﺎرﯾﻮﻓﯿﻠﻦ و 0/018 ﻣﯿﻠﯽﮔﺮم ﮔﺎﻣﺎﺗﺮﭘﯿﻨﻦ وﺟﻮد داﺷﺖ. ﻣﯿﺰان آﻟﮑﺎﻟﻮﺋﯿﺪ ﺗﺎم ﻣﻮﺟﻮد در ﻫﺮ ﻗﺮص، 0/06 ﻣﯿﻠﯽﮔﺮم ﻣﻌﺎدل آﺗﺮوﭘﯿﻦ ﻣﺤﺎﺳﺒﻪ ﮔﺮدﯾﺪ. ﻧﺘﯿﺠﻪﮔﯿﺮي: اﯾﻦ ﻓﺮﻣﻮﻻﺳﯿﻮن ﻃﺒﯿﻌﯽ ﻣﯽﺗﻮاﻧﺪ ﺑﻪ ﻋﻨﻮان ﯾﮏ ﻣﺤﺼﻮل ﻃﺐ ﺳﻨﺘﯽ اﯾﺮاﻧﯽ ﺑﻪ ﺻﻮرت ﺑﺎﻟﯿﻨﯽ ﻣﻮرد ارزﯾﺎﺑﯽ ﻗﺮار ﮔﯿﺮد و ﺑﻪ ﻋﻨﻮان ﯾﮏ ﻣﮑﻤﻞ ﺗﻘﻮﯾﺖﮐﻨﻨﺪهي ﺣﺎﻓﻈﻪ اراﺋﻪ ﺷﻮد.

Alzheimer’s disease (AD) is the most common type of dementia that has been cited in traditional Persian medicine (TPM) manuscripts under the heading "Farāmūshkārī", "Fesād-e-Zekr" and "Nesiān". Since AD is a significant challenge for health professionals, searching for natural and effective compounds is crucial. Objective: Given the importance of AD, this study has provided a standardized plantbased tablet based on TPM. Methods: Related preparations were extracted from leading pharmaceutical manuscripts of TPM. Out of 127 gathered compound formulations, a preparation containing Bunium persicum (Boiss.) B.Fedtsch. (Persian cumin) and Piper nigrum L. (black pepper) was selected from the Canon of Medicine of Avicenna. After evaluating various excipients for tablet preparation, the proportions of Persian cumin (40 %), black pepper (40 %), hydroxypropyl methylcellulose (HPMC), and polyvinylpyrrolidone (PVP) as solution binder (20 %) were chosen. The final formulation was undergone through ethanol and dichloromethane and essential oil extractions to prepare for HPTLC fingerprints and GC assessments, respectively. The total alkaloid in the tablet's ethanol extract was also determined via spectrophotometry. Results: The final tablet was standardized via GC based on trans-caryophyllene (29.22 %) and -terpinene (17.72 %). Each tablet contains an average of 0.041 mg of transcaryophyllene and 0.018 mg of -terpinene. The amount of the total alkaloid in each tablet was determined 0.06 mg, equivalent to atropine. Conclusion: This prepared simple natural formulation can be considered a traditional-based product to be evaluated clinically and presented as a memory enhancer supplement.