تأثير تمرين تناوبي شديد بر بيان ژن هاي آديپونكتين و رزيستين در بافت چربي احشايي موش هاي ديابتي

The Effect of High Intensity Interval Training on the Gene Expression of Adiponectin and Resistin in Adipose Tissue of Diabetic Rats

مقدمه و هدف: تمرين تناوبي با حجم مناسب باعث تنظيم بيان ژن در بيماران مبتلا به ديابت شده و مخاطرات هايپرگلايسمي را كاهش مي­دهد. هدف از اين مطالعه، بررسي تأثير تمرين تناوبي شديد بر بيان ژن­هاي آديپونكتين و رزيستين در بافت چربي احشايي موش­هاي ديابتي مي­باشد. روش كار: پژوهش حاضر از نوع تجربي بود. بدين منظور24 سر موش نر ديابتي نژاد ويستار به سه گروه تقسيم شدند ;( n=8) كنترل سالم (NC)، كنترل ديابتي (DC)، تمرين تناوبي شديد (HIIT). القاء ديابت توسط استرپتوزتوسين پس از 12 ساعت ناشتايي انجام شد. براي سنجش گلوكز پلاسما از روش گلوكز اكسيداز، انسولين با روش الايزا و اندازه­ گيري شاخص مقاومت به انسولين از روش HOMA-IR استفاده شد. جهت تعيين بيان ژن­ هاي آديپونكتين و رزيستين از روش الايزا و مقايسه گروه ­ها توسط آنوواي يك طرفه در سطح 0/05 استفاده شد. يافته ­ها: بيان ژن آديپونكتين در گروه HIIT نسبت به گروه DC افزايش معناداري داشت (0/05

Introduction: Moderate volume interval training regulates gene expression in patients with diabetes and reduces the risks of hyperglycemia. The aim of this study was to evaluate the eeffects of high intensity interval Training on the gene expression of Adiponectin and Resistin in adipose tissue of diabetic Rats. Materials and Methods: The present study was experimental. To this end, 24 diabetic male Wistar rats were divided into three groups (n-8); normal control (NC), diabetic control (DC), high intensity interval training (HIIT). Diabetes was induced in all groups expect for normal control group by intraperitoneal streptozotocin injection after 12 hours of fasting. Glucose oxidase was used to measure glucose in plasma using ELISA method to measure insulin levels.HOMA-IR method was used to measure insulin resistance index. Real-time PCR technique was used to evaluate the gene expression of the Adiponectin and Resistin. Comparison of groups was performed by (one way ANOVA) at the alpha level of 0.05. Results: Adiponectin gene expression was significantly increased in HIIT group compared to DC group (p<0.05). Resistance gene expression in HIIT group was significantly reduced compared to DC group (p≤0.001). Insulin resistance was significantly reduced in the HIIT group. Conclusion: The results showed that intense intermittent exercise reduced insulin resistance and possibly regulated cellular metabolism by increasing adiponectin gene expression and decreasing resistin gene.