تأثير تمرين هوازي و مكمل كوركومين بر سطوح رنالاز، كراتينين و نيتروژن اورة خون و آسيب كليوي در موش‌هاي صحرايي نر روش القايي سكتة قلبي

Effects of Aerobic Training and Curcumin Supplementation on Blood Renalase, Creatinin and BUN Levels and Renal Damage in Male Rat Model of Induced Myocardial Infraction

: سرعت آسيب كليه پس از انفاركتوس قلبي به‌سرعت افزايش مي‌يابد، اما تمرينات هوازي و مكمل كوركومين پس از سكتة قلبي تأثيرات بسيار مثبتي بر كليه و قلب دارند. همچنين رنالاز خون مي‌تواند اطلاعات مناسبي از وضعيت سلامت هر دو اندام فراهم كند. بنابراين هدف پژوهش حاضر بررسي تأثير تمرين هوازي و مكمل كوركومين بر سطوح رنالاز، كراتينين نيتروژن اورة خون (BUN) و آسيب توبولي كليه در موش‌هاي صحرايي نر روش القايي سكتة قلبي بود. مواد و روش‌ها: 40 موش صحرايي نژاد ويستار به‌طور تصادفي به پنج گروه (هشت‌تايي) شامل سالم-كنترل، سكته-كنترل، سكته-تمرين، سكته-كوركومين و سكته-توأم (شامل تمرين و كوركومين) تقسيم شدند. سكتة قلبي از طريق تزريق ايزوپروترنول القا شد. تمرين طي هشت هفته به‌صورت پنج جلسه دويدن در هفته با شدت متوسط روي نوار گردان انجام گرفت و كوركومين نيز (mg/kg.day) 15 به‌صورت گاواژ مصرف شد. مقدار رنالاز خون به روش الايزا، مقدار كراتينين و نيتروژن اورة خون به روش آنزيمي و شدت آسيب بافتي به روش هيستولوژيك تعيين شدند و داده‌ها با تحليل ‌واريانس‌ تكراهه (آزمون تعقيبي توكي) و آزمون خي‌دو در سطح اطمينان 95 درصد تحليل شدند. نتايج: در گروه سكته كنترل، مقدار رنالاز (001/0P=)، كراتينين (001/0P=) و BUN(025/0P=) خون به‌طور معناداري بيشتر از گروه كنترل سالم بود، اما در هر سه گروه مداخله شامل سكته-تمرين، سكته-كوركومين و سكته-توأم، مقدار اين سه شاخص با گروه سالم كنترل تفاوت معناداري نداشت (05/0

The risk of renal injury rapidly increases following myocardial infraction (MI), however; aerobic training and curcumin supplementation have beneficial effects on both the kidneys and the heart post MI. Moreover, blood renalse level can also provide appreciable information regarding these organs health status. Therefore, the aim of study was to investigate the effects of aerobic training and curcumin supplementation on blood renalase, creatinin and blood ure nitrogen (BUN) levels and renal tubular damage in male rat model of induced myocardial infraction. Material and Methods: 40 male wistar rats were randomized into five groups (n=8) of healthy control, MI control, MI training, MI curcumin and MI concomitant (training+curcumin). MI was induced by isoproterenol injection. The training program (consisted of five sessions of running/week at moderate intensity on a treadmill) was conducted throgh eight weeks and curcumin was administrated by gavage method 15 mg/kg.day. Blood renalse was determined by ELISA method, while creatinine and BUN level by enzymatic method and the tissue damge level was evaluated by histologic analysis. The data was analysed by one-way ANOVA (Tokey test as post hoc) and Chi square tests with a confidence level of 95%. Results: Blood renalse (P = 0.001), creatinine (P = 0.001) and BUN (P = 0.025) was significantly higher in MI control group compared to healthy controls. However, the amounts of these indices had no significant differences with MI control group in all three intervention groups of MI training, MI curcumin and MI concomitant (P > 0.05). However; renal injury was significantly lower in all three intervention groups than MI control group (P = 0.007, P = 0.037 and P = 0.001 respecetively) and also in MI concomitant group compared to both of MI training (P = 0.006) and as well as MI curcumin (P = 0.003) groups. Conclusion: The hazardous effects of MI on all three variables could be ameliorated following all three interventions. However, the concomitant intervention is superior to both of the aerobic training as well as curcumin supplementation for reductions in the histologic injury level.