ﻣﺪلﺳﺎزي ﺑﺎر ﻣﻮﻟﺪﻫﺎي ﺗﻮان ﭘﺎﻟﺴﯽ در ﮐﺎرﺑﺮد اﻟﮑﺘﺮوﭘﻮرﯾﺸﻦ ﺑﺎ اﺳﺘﻔﺎده از ﻃﯿﻒﺳﻨﺠﯽ اﻣﭙﺪاﻧﺲ ﺳﻠﻮلﻫﺎي ﻧﺮﻣﺎل و ﺳﺮﻃﺎﻧﯽ رﯾﻪ اﻧﺴﺎن

Load Modeling of The Pulsed Power Generators for Electroporation Using Impedance Spectroscopy of Human Lung Normal and Cancer Cells

در اﯾﻦ ﻣﻘﺎﻟﻪ ﺑﻪ ﻣﺪلﺳﺎزي ﺑﺎر ﻣﻮﻟﺪﻫﺎي ﺗﻮان ﭘﺎﻟﺴﯽ ﺟﻬﺖ ﮐﺎرﺑﺮد اﻟﮑﺘﺮوﭘﻮرﯾﺸﻦ و ﺑﺎ اﺳﺘﻔﺎده از ﻃﯿﻒﺳﻨﺠﯽ اﻣﭙﺪاﻧﺲ ﺳﻠﻮلﻫﺎي ﻧﺮﻣﺎل و ﺳﺮﻃﺎﻧﯽ رﯾﻪ اﻧﺴﺎن ﭘﺮداﺧﺘﻪ ﺷﺪه اﺳﺖ. ﺑﺎ وﺟﻮد ﺗﻤﺎﯾﺰاﺗﯽ در رﻓﺘﺎر اﻟﮑﺘﺮﯾﮑﯽ ﺳﻠﻮلﻫﺎي ﻧﺮﻣﺎل و ﺳﺮﻃﺎﻧﯽ، ﻣﺪلﺳﺎزي اﻟﮑﺘﺮﯾﮑﯽ ﺳﻠﻮلﻫﺎ ﻣﯽﺗﻮاﻧﺪ در ﺗﺸﺨﯿﺺ ﺳﺮﻃﺎن و اﯾﺠﺎد اﻧﺘﺨﺎب ﭘﺬﯾﺮي در درﻣﺎن )ﻣﺮگ در ﺳﻠﻮلﻫﺎي ﺳﺮﻃﺎﻧﯽ و ﻋﺪم آﺳﯿﺐ ﺑﻪ ﺳﻠﻮلﻫﺎي ﻧﺮﻣﺎل( ﻣﺆﺛﺮ ﺑﺎﺷﺪ. ﻫﻤﭽﻨﯿﻦ ﺑﺎ ﺗﻮﺟﻪ ﺑﻪ ﺗﻐﯿﯿﺮات ﮔﺴﺘﺮده اﻣﭙﺪاﻧﺲ ﺳﻠﻮلﻫﺎ ﺗﺤﺖ ﺷﺮاﯾﻂ ﻣﺨﺘﻠﻒ و واﺑﺴﺘﮕﯽ ﺳﺎﺧﺘﺎرﻫﺎي ﻣﻮﻟﺪﻫﺎي ﭘﺎﻟﺴﯽ ﺑﻪ اﻣﭙﺪاﻧﺲ ﺑﺎر، ﻣﺪلﺳﺎزي ﺑﺎر ﻣﯽﺗﻮاﻧﺪ در ﻃﺮاﺣﯽ ﻣﻮﻟﺪﻫﺎي ﭘﺎﻟﺴﯽ ﺑﺮاي اﯾﺠﺎد ﭘﺎراﻣﺘﺮﻫﺎي ﭘﺎﻟﺲ ﺑﺎ ﺑﯿﺸﺘﺮﯾﻦ اﺛﺮﮔﺬاري، ﺑﺴﯿﺎر ﻣﻔﯿﺪ ﺑﺎﺷﺪ. در اﯾﻦ ﻣﻘﺎﻟﻪ، اﻣﭙﺪاﻧﺲ ﻧﻤﻮﻧﻪﻫﺎي زﯾﺴﺘﯽ واﻗﻊ در ﮐﻮوتﻫﺎي اﻟﮑﺘﺮوﭘﻮرﯾﺸﻦ در ﻓﺮﮐﺎﻧﺲﻫﺎي ﻣﺨﺘﻠﻒ اﻧﺪازهﮔﯿﺮي ﺷﺪه و درﻧﻬﺎﯾﺖ ﺑﻪ ﺟﻬﺖ ﺗﺠﺰﯾﻪوﺗﺤﻠﯿﻞ ﮐﻤﯽ وﯾﮋﮔﯽﻫﺎي اﻟﮑﺘﺮﯾﮑﯽ ﺑﺎر، ﯾﮏ ﻣﺪار ﻣﻌﺎدل اﻟﮑﺘﺮﯾﮑﯽ دﻗﯿﻖ اراﺋﻪ ﻣﯽﮔﺮدد. درواﻗﻊ ﻣﺪل اﻟﮑﺘﺮﯾﮑﯽ اراﺋﻪ ﺷﺪه، ﻣﺘﻤﺮﮐﺰ ﺑﻪ ﺳﻠﻮلﻫﺎ ﻧﺒﻮده و ﺑﺮاي ﻣﺠﻤﻮﻋﻪ ﮐﻮوت و ﻣﺤﺘﻮﯾﺎت داﺧﻠﯽ آن اﺳﺖ. اﯾﻦ ﻣﺪل ﺑﺎ در ﻧﻈﺮ ﮔﺮﻓﺘﻦ اﺛﺮ ﺧﺎزن دوﻻﯾﻪ اﻟﮑﺘﺮﯾﮑﯽ و اﺛﺮات ﭘﺎرازﯾﺘﯿﮏ و ﺑﺴﻂ ﺑﺎزه ﻓﺮﮐﺎﻧﺴﯽ از 100 ﻫﺮﺗﺰ ﺗﺎ 500 ﻣﮕﺎﻫﺮﺗﺰ اراﺋﻪ ﺷﺪه اﺳﺖ. ﻧﺘﺎﯾﺞ ﻧﺸﺎن ﻣﯽدﻫﺪ، ﻣﺪل اﻟﮑﺘﺮﯾﮑﯽ اراﺋﻪﺷﺪه ﺑﺎ دﻗﺖ ﻧﺴﺒﺘ ﺎً ﺧﻮﺑﯽ، ﺑﺎ اﻧﺪازهﮔﯿﺮيﻫﺎي اﻣﭙﺪاﻧﺲ اﻧﺠﺎمﺷﺪه ﺑﺮاي ﻧﻤﻮﻧﻪﻫﺎي ﻣﺨﺘﻠﻒ ﻣﻄﺎﺑﻘﺖ دارد.

In this paper, the load of the pulsed power generators is modeled using the impedance spectroscopy of human lung normal and cancer cells for electroporation. Due to the differences in the electrical behavior of normal and cancer cells, cell modeling can be used in cancer diagnosis and treatment selectivity (causing cell death in cancer tissues and not damaging the cells, and normal tissues) is effective. Also, due to the wide changes in cell impedance under different conditions and the dependence of pulse generator structures on load impedance, cell modeling can have a significant effect on the design of pulse generators and the creation of pulse parameters with the greatest effect. In this paper, the impedance of biological samples is measured at different frequencies. Finally, for quantitative analysis of the electrical properties of cells, an accurate electrical equivalent circuit is provided for the load. The proposed electrical model is not cell-focused but on the set of cuvette and its internal contents. This electrical model, taking into account the effect of the two-layer electrical capacitor and parasitic effects, and expanding the frequency range from 100 Hz to 500 MHz. The results show that the equivalent electrical circuit provided in most cases with an low error is consistent with the impedance measurements performed for different samples