در نسل دوم كلزاي تراريخت CAMV 53S و ميزان فعاليت پروموتور GUS برريس واراثت ژن

Analysis of GUS Gene Inheritance and CaMV 35S Promoter Activity in Second Generation of Transformed Canola

The GUS gene was used to assess caMV 35S promoter activity and to optimize an efficient transformation system for canola (Brassica napus). Second generation of transformed canola plants containing kanamycin resistant (KanR) and GUS gene linked to the CaMV 35S promoter were used to histochemical analysis the 35S promoter functions. The GUS inheritance was tested using PcR technique and kanamycin resistant gene. The majority of the seedlings (more than 70%) were kanamycin resistant. Plant segregation pattern for kanamycin resistance revealed that some of the transformants contained more than one T-DNA. Tissue samples from different parts of Transgenic plants including callus, shoots and flowers were tested by histochemical analysis. The GUS gene , controled by 35S promoter was expressed continuously in different parts of the transgenic plants as well as during different developmental stages (before organogenesis, after regeneration and during reproduction). The GUS gene expression studies showed that CaMV 35S promoter is a strong promoter which can express the transformed gene in different tissues at different developmental stages in canola.