اثر داروي دپرينل بر سطح سيناپتوفيزين و استيل كولين ترانسفراز پس از انجام آكسوتومي عصب سياتيك در موش صحرايي

The Effect of Deprenyl on Expression Synaptophysin and Choline Acetyltransferase in Rat after Sciatic Nerve Axotomy

The neuroprotective effects of deprenyl in motoneuron of spinal cord after axotomy peripheral nerve such as sciatic is reported, it is a mono amino axidase type-B (MAO-B) inhibitor. The neuron function is the release of neurotransmitters from pre-synaptic vesicle to synaptic cleft. One of the neurotransmitter is acetylcholine which is synthesized by choline acetyltransferase (Chat) and is used as a marker for function. Synaptophysin is one of synaptic vesicle protein and is used as a marker for neurotransmitter. The aim of this research is to study the effect of deprenyl (2.5mg/kg) injection on synaptophysin and Chat immunoreactivity. Intra peritoneal injection was done following axotomy and continued for 21 days, they perfuse with paraformaldehhyde (4%) and spinal cord segment L4-L6 processed for immunohistochemistry. The results showed that deprenyl (treated) axotomized had higher Chat and synaptophysin expression as compared with those untreated axotomized.