تاثير درجات مختلف آبزدايي بر سطوح پلاسمايي كراتين كيناز و لاكتات دهيدروژناز كشتي گيران حرفه اي شهر اهواز

The Effect of Different Degrees of Dehydration on Plasma Creatine Kinase and Lactate Dehydrogenase Levels of Professional Wrestlers in Ahvaz

با وجود اثبات اثرات منفي كاهش وزن بر اثر آب زدايي، اين روش هنوز هم در بين ورزشكاران رايج است. هدف از انجام تحقيق حاضر بررسي تاثير آب زدايي با درجات مختلف بر سطوح سرمي آنزيم هاي نشانگر آسيب عضلاني كشتي گيران حرفه اي شهر اهواز بود. از بين كشتي گيران حرفه اي شهر اهواز، پس از غربال گري تعداد 40 نفر انتخاب و به صورت تصادفي ساده در سه گروه تجربي و يك گروه كنترل تقسيم شدند. گروه هاي تجربي در سه سطح خفيف، متوسط و شديد متحمل آب زدايي شدند. سطوح پلاسمايي شاخص هاي آسيب عضلاني يعني آنزيم هاي كراتين كيناز (CK) و لاكتات دهيدروژناز(LDH) به روش كينتيك آنزيمـي اندازه گيري شد. جهت مقايسه درون گروهي از t وابسته و مقايسه بين گروهي از آزمون آنوا در سطح معني داري 0.05 استفاده شد. نتايج نشان داد كه سطوح پلاسمايي كراتين كيناز، در گروه هايي كه آب زدايي متوسط و شديد داشتند و سطوح لاكتات دهيدروژناز فقط در گروه آب زدايي شديد افزايش معني دار داشت(0.05>P). همچنين در مقايسه بين گروهي سطوح كراتين كيناز مشاهده شد كه بين گروه آب زدايي شديد با ديگر گروه ها و نيز گروه آب زدايي متوسط و ديگر گروه ها تفاوت معني داري وجود دارد (0.05>P). ولي بين دو گروه آب زدايي متوسط و شديد تفاوت معني داري مشاهده نشد (0.05P) و تفاوت ديگر گروه ها با هم معني دار نيست (0.05

Despite the proving of the negative effects of dehydration, many athletes use of this strategy yet. The aim of this study was to investigate the effect of dehydration with different degrees on serum levels of enzymes biomarker muscle damage in professional wrestlers in Ahvaz city. Among the professional wrestlers in Ahvaz city, after screening, 40 wrestlers were selected and randomly divided into three experimental groups and one control group. Experimental groups underwent dehydration at three levels of mild, moderate and severe. The plasma levels of Creatine Kinase (CK) and Lactate Dehydrogenase (LDH) were measured by enzyme kinetics method. for intragroup comparison and intergroup comparison, were used of the dependent t-test and analysis of variance (ANOVA) test respectively at a significance level of 0.05. The results showed that plasma CK levels increased significantly in the moderate and severe dehydration groups and LDH levels increased only in the severe dehydration group (P <0.05). Also, in the intergroup comparison of CK levels, it was observed that there was a significant difference between the severe dehydration group and other groups, as well as the moderate dehydration group and other groups (P <0.05). But no significant difference was observed between the moderate and severe dehydration groups (P <0.05). Also, the results of comparison between groups of LDH showed that only the difference between the severe dehydration group with other groups was significant (P <0.05) and the difference between the other groups was not significant (P <0.05). According to the results of this study, it is suggested that to prevent the possibility of muscle damage and consequently reduced the exercise performance, avoided the dehydration of more than 1% in one session and if necessary, dehydrate in more sessions and for longer.