بررسي اثرات ناهنجاري زايي تجويز همزمان الكل و آسپيرين در تكامل سيستم اسكلتي جنين موش سوري

Teratogenic effects of synchronous administration of Alcohol and Aspirin on development of skeletal system in mouse embryo

Abnormalities caused by alcohol are very extensive and are called fetal alcoholic syndrome(FAS).There are some hypotheses about alcohol and pathological mechanisms of its effect. Based on one of the recent hypotheses,alcohol consumption can change maternal and fetal prostaglandin levels. On the other hand a strong prostaglandin inhibitor is Aspirin.Investigations which has done on mouse that received alcohol and aspirin together,showed decrease in limbs length and kidney abnormalities in their offsprings. Others showed that Aspirin had no inhibition on alcohol adverse effects. The aim of present study is to determine the effects of administration of alcohol and Aspirin together.135males and females ( 1 male versus 3 female) chose randomly divided in 5 groups. After vaginal plaque observation in 8th day of gestation , controls received nothing while other groups received suitable volumes of physiological serum, alcohol, Aspirin., and a combination of alcohol and Aspirin respectively by in.traperitoneal injection. In 18th day ofʹ gestation , animals were killed by cervical dislocation . Embryos were collected , gross skeletal system abnormalities were studied. There after staining was done by Alizarin Red and Alcian blue and ossification centers were counted in each group. Results showed that there are lower height and weight, and more skeletal system abnormalities in group that received a combination of alcohol and Aspirin compared with the group that received alcohol alone. Thus administration of Aspirin with alcohol does not decrease frequency of skeletal abnormalities and probably there must be other factors, except prostaglandin, involved in skeletal system abnormalities.