بررسي مقايسه اي مقاومت استافيلوكوك هاي كوآگولاز مثبت به آنتي بيوتيك هاي بتالاكتام

Relative frequency study of coagulase positive staphylococci resistance to betalactam antibiotics using iodometric and acidometric assays

During period of five month study 38 coagulase positive staphylococcus isolates were identified from various clinical specimens from 600 patients at the AL-Zahra university hospital in Isfahan. Betalactamase production assays using rapid acidometric and iodometric tests showed that 78.9% and 73.6% of isolates were positive respectively . The difference in determination rate between acidometric and iodometric tests was not statistically significant. Moreover the acidometric test was cheaper and more easy to perfrom than iodometric test. In vitro sensitivity testing using the disc diffusion method showed that all of isolates were resistant to carbenicillin , ampicllin and amoxicillin , while 7.9% and 13.2% were resistant to cefazolin and cephalexin respectively . Therefore we recommend use of cefazolin, cephalexin and oxacillin for treatment of patients with staphylococcus infections.