RP-HPLC پيش بيني بازداري برخي داروهاي ضد صرع در فازهاي متحرك دو حلالي در

Prediction of Retention of some anti-epileptic drugs in binary solvent mobile phases in RP-HPLC.

Capacity factors of phenobarbital, phenytoin and carbamazepine in mobile phases containing water and the organic modifiers: methanol, acetonitrile, acetone and tetrahydrofuran were determined and the obtained results fitted to the proposed model: p Inky = J{§{ + J2§2 +JX§2 ?p B1(§{ -2§2 )ʹ where kʹ is the capacity factor of the analyte, §x and §2 are the volume fractions of solvents 1 and 2, J,, J2 and B, are the model constants. Average percentage deviation (APD) was computed as an accuracy criterion for the proposed model, the linear model of Schoenmarkers and the cubic equation of Jandera and the obtained mean and standard deviation of APDs were 14,6±10/79 42.3±11.2 and 32.7±17.8 %, respectively. The accuracy of the models was also checked using collected data from the literature and the calculated values were 1.64+0.85, 22.11 ± 2.48 and 4.87±1.98 %, respectively. The results showed that the proposed model provided more accurate computations in comparison with the previously published models and the mean differences were statistically significant.