بررسي اثر ريز نمونه و سطوح مختلف اكسين و سيتوكينسين بر ريخت زايي سويا

Effect of explant and various levels of auxin and cytokinine on soybean regeneration

In order to select the best explant and suitable medium for organogenesis in soybean, two seperate experiments were performed 1) effect of explant (epicotyl, hypocotyl, cotyledonary node, first leaf including cotyledonary node, leaflet, leaf, immature embryogenic axis, immature embryo and mature embryognic axis) and regeneration medium (MS medium with 1.5 mg/I BA and 0.5 mg/I IAA or 1.5 mg/I BA and 0.5 mg/I NAA, 3% sucrose and 0.8% agar) and 2) effect of different rates of BA (0.5, 1 and 2 mg/I) and IAA ( 0,5 and 1 mg/I) on organogenesis and mass callus production. Results of first experiment indicated that the response of explants to regeneration rnedium was very different and two regeneration media had significant differences in regeneration. However, cotyledonary node and the first leaf including cotyledonary node on medium with BA and IAA had the highest shoot formation rate ( 77 and 70%) and shoot number (2.8 and 2.4), respectively.There were no significant differences between these two media in mass callus production, whereas there were significant differences among explants and cotyledonary node with leaflet had the highest mass callus production. On the basis of the second experiment, the best amount of BA and IAA were 1-2 mg/I and 0.5-1 mg/I respectively.