مطالعه ميزان پروتيين و تفكيك زنجيره هاي كازييني در شير چهارگونه پستاندار به روش الكتروفورز ژل آگاروز

Investigation of quantification of protein and fractionation of casein chains of milks produced by four mammal species using Agarose Gel Electrophoresis

The quantification of protein in four mammalsʹs milk including cow, buffalo, sheep and goat using lowry method based on the measurment of the colour intensity iʹarmed during reaction between phenolic amino acids and folin-phenol reagent by spectrophotometer at 750 nm, showed highest amount of protein in sheep and lowest in goatʹs milk. The results of quantification of different fractions of caseins in above milk samples using Agarose Gel Electrophoresis (AGE) technique showed maiximum amount of ct-casein in cowʹs milk with corresponding minimum /t-casein fraction. The highest amount of /t-casein was found in goatʹs milk with corresponding minimum a-casein. No significant differences were observed in n-casein fractions in four different kinds of milks.