كليدواژه :
Alkaloids , متابوليت هاي ثانويه , داتوره , آلكالوييد , كشاورزي , Secondary metabolites , آگروباكتريوم , Transformed hairy roots , Agrobacterium rhizogenes , ريشه مويين تراريخت , datura , ريزوجنز
چكيده لاتين :
Secondary metabolites have an important role in adaptation of plants to their environment and are the main sources of pharmaceutical materials such as alkaloids. Many of these compounds are produced through chemical synthesis for many years, while most of them obtained from natural resources due to their complicated structures. Production of secondary metabolites via tissue culture has some advantages over traditional methods. Alkaloids are first synthesized in roots and then transported to shoots and leaves. Hairy root cultures could be employed artificially to produce these metabolites. Hairy roots could be induced using Agrobacterium rhizogerres. En this research, explants of leaves and shoots of Datura. stramonium were inoculated with A .rhizogenes strain A4 in B5 medium. The nature of transgenic hairy roots was examined using PCR with specific primers for ro1B and roiC of T-DNA. Growth and rate of alkaloids of transformed hairy roots were compared with normal ones using a completely randomized design. Rates of hyoscyamine and scopolamine alkaloids were measured through HPLC machine. The results indicated that the growth of transformed hairy roots was stable over time and their yield was more than three folds of that of normal ones. The results of HPLC showed that the rates of alkaloids did not change in transformed hairy roots compared to those of normal hairy roots. As a result, it is concluded that the presence of Ri plasmid in transformed hairy roots increases the content of hyoscyamine and scopolamine alkaloids.
