تجزيه زيستي آنتراسن در محيط كشت فوق اشباع توسط كشت مخلوط

Biodegradation of Anthracene in Supersaturated Medium by Contaminated Soil Micro-organisms Mixed Culture

Soil contamination with petroleum hydrocarbons(TPH) such as aromatic hydrocarbons is one of the major problem that environment is facing today. In this research, petroleum contaminated soils around the Tehran Refinary have been examined to study the anthracene biodegradation rate using different microorganisms. Inoculum of microorganisms were adapted in mineral mediums with four types of carbon sources (Anthracene,Naphtalene,Toluene or mixture of all) in separate reactors. Grown microorganisms in reactors were named mixA, mixN, mixT and mixANT based on carbon sources. Biodegradation of anthracene was investigated by using above mentioned group of microorganisms. Cells of microorganisms were inoculated in liquid mineral medium at 0.65 and 70 mg/l of anthracene for the investigation of biodegradation potential. Mix N showed 100%, and 98% respectively removal for 0.65 and 70 mg/l. Then mix N was selected for the investigation of biodegradation rate. Specific biodegradation rate of different concentration of anthracene by mix N was determined in supersaturated mediums of anthracene. Community of inoculated microorganisms in all concentrations of this stage was 9X10^7 N/100ml and specific biodegradation rate of 1, 5, 10, 50 and 100 mg/I of anthracene were 0.0514, 0.0686, 0.0563, 0.1238 and 0.0384 day^-1 respectively.The highest specifice biodegradation rate was achieved at a level of 50 mg/l.