بر روي موش هايي كه كتوكونازول دريافت كرده اند ANP بررسي اثرات فيزيولوژيك عصاره پپتيد تاتريورتيك دهليزي

Physiologic effects of atrial natriuretic peptide on rats receiving ketokenazol

Background: Atrial natriuretic peptide (ANP) is a 28 amino acid hormone secreted by atrial monocytes in response to local wall stretch.. Recent studies have shown that glucocorticoidcs can express ANP genes and elevate its level. Ketokenazol is an antiadrenal drug used to reduced glucocorticoides level, thus may result in reduction in ANP level. Thus, the present study was conducted to determine the physiologic effects of ANP on rats receiving ketokenazol. Materials and methods: Human atria were used for ANP. For ANP extraction preparation, tissue was boiled in specific solutions to inactivate intrinsic protease. Then cooling was carried out at 4 degrees centigrade and homogenization and centrifugation were occurred. PH was regulated using dialysis procedure. Results: Pulse, systolic and diastolic pressures were revealed to be higher in ketokenazol group. This may be due to reduction in vascular compliance. Injecting human atrial extract may decrease blood pressure by a slow slope. Conclusion: ANP may be safely used in atherosclerotic patients. Further clinical trials are suggested.