بررسي اثر 17- بتااستراديول بر روي بهبهود زخم در موش هاي صحرايي فاقد تخمدان

Effect of 17 beta-estradiol on wound healing in overiectomized rats

Introduction: In addition to their actions on reproductive function, estrogen has important effect on neovascvlarization, proliferation of cells and increase of growth hormone secretion. In this study, the possible effects of 17-b estradiol on cutaneous wound repair in ovariectomized rats were evaluated. Materials and Methods: This experimental study performed on eighty adult female rats. After anaesthesia, all animals were ovariectomized, and they were randomly divided into 6 groups (n = 12 in each group). Group I and II (control groups) received daily topically or subcutaneous injections of 0.1 ml of sesamin oil (vehicle), respectively. Group III and IV treated once dialy with 17-b estradiol valerate ointment (10, and 25 mg/kg, respectively). Group V and VI recevied daily subcutaneous injection of 17-b estradiol valerate on the day of wounding. After aneasthesia, full thickness skin of upper dorsal part of the rats removed in 3 cm2 area. Wound surface area measured in 1, 4, 7, 10, 13, 16, 19, 22 and 25 post-operative days. The time required for complete healing was also recorded. Results: The results show that mean of surface area of wound in all groups at the 1st day were similar. Surface area in group IV at 4th, and 7th day was more than groups I (P< 0.001) and III (P<0.01), and also at 10th day was more than group III (P<0.05). Surface area mean of wound in group II at 16th, 19th, and 22th was less than group V (P<0.01). There are no significant differences between percentage of wound healing in all groups at all postopertive days as compared to control. The time required for compelete healing in treatment groups was more than control groups, but these differences were not significant. In other hand, this time in group II (16.5± 0.42) was less than group I (21.5± 0.8, P<0.05). Conclusion: These data suggest that estradiol particulary, topical application of high dose at the primary days and low dose injection at the end days of wound healing period can impair wound healing.