مطاعه لكتين هيستوشيميايي تغييرات تكاملي گليكوكانژوگيت ها در سلول هاي نوتوكورد در اوايل دوران مورفوژنز در موش

LECTIN HISTOCHEMISTRY STUDY OF DISTRIBUTION OF GLYCOCONJUGATES ON THE NOTOCHORDAL CELLS DURING EARLY MORPHOGENESIS IN MOUSE EMBRYOS

Background & Aim: Notochord is an embryonic mesodermal derived axial structure. In addition of its supporting role, it has multiple inducing abilities to all nearby developing structures including; neural tube, somites and endoderm. The purpose of this study was to characterise the glycoconjugate contents in the components of notochordal process as well as its surrounding mesenchyme during embryogenesis. Materials & Methods: Paraffin fixed 6 mm sections of days 10 to 14 of mouse embryos were processed for histochemical studies by using four horse radish peroxidase labelled lectins, maclura pomifera (MPA) and wistaria floribunda (WFA), lotus tetragonolobus (LTA) and ulex europeus (UEA1) that are specific for N-acetylgalactosamine (D-Gal NAc) and a-L-Fucose side chains of the glycoconjugates respectively. Results: Our results revealed that a-L-fucose might have a role in the notochord development, but not on mesenchyme differentiation. Furthermore notochordal cells and adjacent mesenchyme with their extracellular matrix showed extensive reaction by using MPA and WFA lectins which are specific for Gal NAc. Therefore this sugar might has role in the notochord development and nearby mesenchyme differentiation. Conclusion: The expression of the Gal NAc and a-L-fucose as terminal sugars on glycoconjugates moieties are stage-dependent and thus probably genetically regulated. The timing and distribution of lectin reactions suggest that these molecules (Gal NAc and Fucose) may play a role (s) in notochordal interactions with adjacent mesenchyme and subsequent formation.