توليد و خالص سازي اينترفرون لوكوسيت گاوي مقاوم اسيد

PRODUCTION AND PURIFICATION OF AN ACID-STABLE BOVINE LEUKOCYTE INTERFERON

Background & Aim : Bovine leukocyte interferon (IFN) was produced in bovine peripheral blood leukocytes after priming and induction with Sendai virus. This interferon can be used in bovine viral infection and for this reason, it is necessary to have a suitable procedure for interferon purification. Materials & Methods: Interferon produced with Sendai virus by bovine leukocytes was precipitated with KSCN (pH 3.5) and purified by column chromatography using Sephadex G-100. To obtain higher recovery of interferon several procedures were used. Results : Using 25% ethylene glycol and one molar NaCl in the column elution buffer resulted in over 93% recovery of the applied IFN activity, versus only 25% when buffer contained no ethylene glycol. Column purified IFN was analyzed by SDS-PAGE in denaturing buffer and a single band of 19000 Dalton protein with interferon activity was identified. Discussion : This is a suitable procedure for purification of enough interferon to be used for production of monoclonal antibody and for biological investigation.