تجسس ميكروآبينوري در افراد ديابتيك با استفاده از روش ساده آگلوتيناسيون غيرمستقيم

DETECTION OF MICROALBUMINURIA IN DIABETIC AND NONDIABETIC PATIENTS USING A SIMPLE LATEX AGGLUTINATION TEST

Introduction : The level of Albumin excretion increase urine in diabetic patients. This amount is not in ordinary detection limit for urine proteins (50mg124 hrs) and it is called microalbuminuria. Methods and materials : A suspension of (I%) human albumin and polystern particles (latex) was made and tests carried out by indirect agglutination and turbidometty methods using proper polyclonal antibodies. Results : The obtained results indicated the detection limit of < 25mgll of albumin in 144 urine samples (diabetic patients, healthy persons and normal urines with added albumin. Discussion : Presence of agglutination indicates a content of albumin level lower than 25mg/l (cut-off), level and lack of agglutination indicates albumin in samples equal or greater than 25mgIL, and denotes the presence of albumin in diabetic patints.