شناسايي گونه و زيرگونه هاي ليشمانياهاي جدا شده از بيماران كالاآزار در شمال غرب ايران

SPECIES & STRAINS IDENTIFICATION OF LEISHMANIA ISOLATED FROM KALA - AZAR PATIENTS IN NORTHWEST OF IRAN

Background & Aim : Leishmaniasis is a general name given to disease caused by any member of protozoan genus Leishmania and can be divided into visceral (kala-azar), cutaneous and mucosal syndromes. For epidemiologic studies, identification of strains & species of lesihmania protozoan in any endemic area is a necessity. The aim of this study characterization of leishmania isolate from patient with kala-azar based on isoenzymes electrophoretic patterns & determination of the main reservoir, deduction from comparison of isoenzyme pattern of reservoir & patients in northwest of Iran were undertaken. Materials & Methods : Twenty one bone marrow aspirated were obtained from patients and after inoculation in semisolid sloppy evaneʹs N.N.N meida electrophoresed by a thin-layer starch gel method for twelve isoenzymes. Results : The isoenzyme profiles of all patients were indistinguishable from L. Infantam LON-49 = [MON-1] zymodeme (MHOM/TN/80/IPTl) and are the same profiles which previously were identified in naturally infected dogs in northwest of Iran. So while the specie) of leishmania was proved. The main reservoir also was identified. Discussion : This study again proved that kala-azar endemic area in northwest of Iran Ls a Mediterranean site of kala-azar.