در تفكيك تومورهاي حد وسط موسينوس و سروز تخمدان از انواع خوش خيم و بدخيم آنها AGNOR بررسي ارزش رنگ آميزي

EVALUATION OF AgNOR STAINING IN DIFFERENTIATION BETWEEN BORDERLINE MUCINOUS AND SEROUS TUMORS OF OVARY FROM BENIGN AND MALIGNANT TYPES

Background & Aim : Differentiation between borderline serous and mucinous ovarian tumors from benign and malignant counterparts are occasionally difficult of impossible, by the use of routine H & E histopathology Statining. In this study, differentiation capacity of argyrophilic nucleolar organizer region (AgNOR) staining was evaluated concerning these tumors. Materials & Methods: For this purpose two AgNOR counts were used. The first m AgNOR (mean number of AgNOR) that reflects ploidy and the second pAgNOR (percentage of nuclei with greater than or equal to five AgNOR per nucleus) that correlates with preliferative activity. Forty - six serous ovarian tumors (15 benign, 13 borderline and 18 malignant lesions) and 36 mucinous ovarian tumors (15 benign, 12 borderline and 9 malignant lesions) were selected and the AgNOR and H & E staining carried out. For each tumor, 100 cells were randomly examined for nuclear AgNORs. Results: A stepwise significant increase in pAgNORs and mAgNORs from benign to borderline and malignant serous and mucinous ovarian tumors was found (p< 0.001) except for two overlaps between pAgNOR of benign and borderlines mucinous tumors. Conclusion: This study concludes that the AgNOR counting is a valuable diagnostic criterion for differentiation between borderline serous and mucinous ovarian tumors from benign and malignant counterparts.