بدنبال استفاده از دوزهاي مختلف داروي سيس پلاتين )OV2008( ارزيابي چرخه سلولي سلولهاي كارسينوماي تخمدان انساني

Cell Cycle Analysis of Human Ovarian Carcinoma(OV2008) After Application of Different Doses of Cisplatin

Cisplatin has been used as a chemotherapeutic agent widely. Its cytotoxicity is through binding with DNA, which will cause cell death. Cell cycle arrest death is among hypothesized cellular effects of Cisplatin. In this study, human ovarian carcinoma, (OV 2008) cells have been exposed to two different doses of Cisplatin and cell cycle arrest has been investigated. OV 2008 cells were exposed to lµg/ml (IC50 of drug) and 31.tglml (IC80 of drug) for one hour in RPML medium supplemented with 10% fetal bovine serum (FBS) Medium. Cells were collected 48, 72 and 96 hours after exposure to Cisplatin with trypsinisation. As was shown with DNA content, Cisplatin caused increasing of G2 and S phases as well as decreasing of G1 phase 48 hours after exposure. G2 cell population was decreased from 48 hours to 96 hours time. However, S phase populations remained high until] 96 hours time. Our results revealed that there was not dose related differences in the cell cycle arrest caused by Cisplatin and no differences in the percentages of GI, S and G2 cells exposed to any of l or 3 µg/ml Cisplatin had. As a conclusion, cell cycle arrest pattern of OV 2008 cells exposed to Cisplatin was not concentration dependent and Cisplatin caused S and G2 phase cell cycle arrest .0V2008 cell lines were sensitive to Cisplatin in G2 and S phases without any indication of concentration.