در توده هاي بدخيم و خوش خيم پستاني بيماران مراجعه كننده به بيمارستان حضرت رسول اكرم (ص ) AGNOR مقايسه اندكس حاصل از رنگ آميزي

THE COMPARISON EVALUATION OF AgNOR INDEX IN THE MALIGNANT AND BENIGN BREAST MASSES

Nucleolar organizer regions(NORs) are loops of DNA included ribosomal genes that ribosomal RNA translated of which synthesis proteins. NORs are in short arms of acrocentric chromosomes and associated with argyrophilic proteins, which are seen as black dots, termed "AgNOR", by using colloidal silver (Ag) staining method. AgNOR count in nuclcous have direct relation with cellular proliferative activity. The significance of this study is to evaluate the potential value of AgNOR in differentiating benign from malignant lesions, particullary, on the slide prepared from small biopsy, Frozen section, FNA and touch imprint, that are difficult to definite diagnosis by routin H&E staining. In this study, AgNOR stained on 90 section of paraffin embeded blocks included, 45 benign lesions and 45 malignant lesions. The average number of NORs in benign lesion and malignant tumor were 4.13 and 9.82 respectively. By using t-test and one way ANOVA-test, significant statistical difference is between benign and malignant lesions, so AgNOR staining is a simple, rapid and cheap method in difinite diagnosis of benign from malignant breast lesions.