انسان HSP06 تعيين سطح آنتي بادي ضد پروتيين شوك حرارتي - 06

DETECTION OF ANTIHUMAN HSP60 ANTIBODY IN PATIENTS WITH TYPE I DIABETES

BACKGROUND AND OBJECTIVE: Type I diabetes is a chronic disorder of pancreas. This disorder occurs as a result of autoimmune destruction of insulin producing beta-cells. Long duration before the onset of clinical symptoms, the autoimmune reactions develop. Therefore, a molecular marker to indicate the initiation of immunological reaction is very critical to identify the people at risk and to take appropriate measures. Since Hsp60 is an antigen involved in the pathogenesis of type I diabetes, this investigation was conducted on detecting anti Hsp60 antibody in various immunoglobulin classes and sub-classes. METHODS: In this study 49 recently manifested type I diabetic patients (50 normal control subjects, 23 individuals with high and 22 with low risk for type I diabetes, as deduced from islet antibody status) were included. Seventeen recently manifested type I patients and 15 normal control subjects were analyzed twice with a three-month interval. The serum samples of patients and normal subjects were collected and stored at 20°c until measurement time. Total anti human Hsp60 antibody was determined in serum samples by EL1SA, positive sera were analyzed for lgG or IgA antibody as well as for IgGI and IgG4 sub-classes by ELISA. FINDINGS: The mean concentration of total anti Hsp60 antibody in recently manifested patients and normal subjects were 182.8±218.5 and 142.5+146.6 Au/ml, respectively (p>0.05). Anti Hsp60 antibody concentration means in type I diabetic relative at high risk and relatives at low risk were 83.6 and 133.4 Au/ml, respectively. There was a significant difference between these two mean values. However, the differences between these two values and mean concentration of total anti Hsp60 antibody in normal subjects were not significant. Moreover, the mean of antibody levels determined for IgG, IgA, IgGI and IgG4 to be considered statistically significant. CONCLUSION: According to the results, there is significant spontaneous Immoral immunity to autologous Hsp60 without detectable association to type I diabetes.