سيب زميني جدا شده از مزارع سيب زميني استان خوزستان X و Y خالص سازي ، تهيه آنتي سرم و تشخيص سرولوژيكي ويروس هاي

Purification , antiserum preparation and serological identification of potato Y and X viruses isolated from potato fields of Khuzestan province

The most important viruses of potato are potato viruses Y and X. The purpose of the present study was to purify PVY and PVX isolated from potato fields of Khuzestan province and to prepare an antiserum against each of them in order to develop a sensitive serological test for diagnosis of these viruses. Potato Y and X viruses were isolated from potato fields in Khuzestan and propagated in Nicotiana tabacum cv. Samsun under greenhouse conditions . Both viruses were purified by a modification of the method of McDonald et a1. ,1976 . Rabbits were used to raise polyclonal antisera against each of the purified viruses.The produced antisera had a titer of 128 for PVY and 256 for PVX in microprecipitin test. Enzyme linked immunosorbent assay (ELISA) and dot immmuno-binding assay (DIBA) systems were developed for both viruses, were quite suitable for detecting viruses in infected plants. No reaction was obtained between the antisera and healthy potato sap.