كليدواژه :
Bacteria , پزشكي , contamination , باكتري ها , Cadaver , آلودگي ها , آموزش آناتومي , كاداورها
چكيده لاتين :
To assess the relationship between the spoilage of cadavers of Anatomy departments to microbial contamination, and to confirm the resistance of bacteria and fungi to antimicrobial effects of fixators, A study was undertaken from Oct 1999 to March 2000.
Fixators, the materials used for fixation of the cadavers, as they contain ethanol and formalin. Protect the cadavers tissues from microbial contamination. But sometimes because of low efficiency of fixators on the new mutants of bacteria, cadavers will spoil.
Two cadavers belonging to the Anatomy Department of Urmia Faculty of Medicine school were selected to assess the fixators effects. Samples were from rib, chest skin, extermitis muscle tissue, blood of jugular vein and stool of sigmoid. Frm each tissue one gram as a sample, was taken and mixed with sterile saline and grained in a sterile mortar prepare a homogenic suspension, From each suspension I ml was cultivated in bacteriological and mycological media and incubated at temperatures 37°C and 25°C respectively. To culture anaerobic microorgamisms, 1 ml of suspensions were transferred to thioglycolate medium.
Cultures were allowed to incubate. For 24 to 48 hours for bacteria and _7 to 10 days for fungi. The isolated strains were stainee and undergone some biochemical tests e.g. nitrate reaction, motility, catalase, gelatin hydrolase, ureas and fermentation of succharose, maltose, manitol, arabinose and growth in 5 °C.
Results show the microbial contamination of the tissues except the blood. The isolated Strains. From the two cadavers were B. circulans, B. maccerans, B. alvei, C. hofmani, C. xerosis, C. ulcerns.
