بررسي اثر كربنوكسولون بر ميزان پراكسيداسيون چربي در جريان ايسكمي فراگير مغزي در هيپو كامپ رت

The effect of carbenoxolone on lipid peroxidation level during global ischemic-reperfusion in hippocampal rat slices

Present studies have shown that gap junction channels could be a novel target to reduce brain damage during cerebral ischemia. Carbenoxolone, a gap junction channel blocker decreased the spread of cell death in in vitro brain ischemic model and inhibition of lipid peroxidation has been of much interest to ameliorate excitotoxic neuronal damage. Thus the aim of the present study was to determine the effect of carbenoxolone on lipid peroxidation in experimental global ischemicreperfusion in the rat hippocampus. The brain damages were determined using the measurement malondialdehyde (MDA). Cerebral ischemia was induced by four-vessel-occlusion (4VO). Carbenoxolone (50-200mg/kg), phenytoin (50 mg/kg) and normal saline (10ml/kg) were administered intraperitoneally immediately after reperfusion. The malondialdehyde (MDA) was measured using the thiobarbituric acid (ʹIBA) test. The MDA level was higher in the saline group during ischemicreperfusion than the sham group. The MDA levels recovered significantly upon phenytoin and carbenoxolone (100-200 mg/kg) therapy in the ischemic-reperfusion. These results suggest that carbenoxolone may protect the brain against lipid peroxidation in cerebral ischemia.