چسبندگي هليكو باكتر پيلوري به هفت رده سلولي انساني

Helicobacter pylori attachment to 7 mamalian cell lines(HepII, HeLa, SW742, AGS, HT29/219, HT29, Caco-2)

Helicobacter pylori is the etiologic agent of chronic --active gastritis, gastroduodenal ulcers in humans, and a co-factor in the oceurance of gastric cancer and mucosa-associated lymphoid tumors. Adherence of H. pylori to the gastric mucosa is a critical, initial step in the pathogenesis of the disease. So bacterial adhesion inhibitory agents provide a novel pharmacologic approach to the management of infectious diseases. 22 H. pylori strains, obtained from the antral or duodenal biopsies of 49 patients with dyspepsia, gastritis, gastric ulcer, duodenal ulcer, etc. were assayed by ELISA reader (UPR: Urea Phenol Red) to investigate the diversity of attachment to 7 mamalian cell lines. The concentration of H. pylori and cell suspension, the condition and temperature, can alter the attachment rate. H. pylori can attach to all 7 cell lines. There are no significant differences between 22 H. pylori strains in attachment to cells. The attachment pattern of H. pylori to the cells showed significant reduction respectively from HepII, HeLa, SW742, AGS, HT29/219, HT29 to Caco-2. Best attachment were seen to Hepll, HeLa and SW742 cells, and among these HepII was the best cells for this purpose. Our studies suggest that HepII, HeLa and SW742 cells could serve as a suitable in-vitro model for the study of H. pylori adhesions, attachment, inhibition of attachment and detachment assays and among these HepII cell is preferably recommended.