بررسي خاصيت آنتي موتاژني فراكسيون هاي عصاره گياه نوروزك

Antimutagemic activity of different fractions of Salvia leriifolia extract

Salvia leriifolia is an Iranian native plant that grow in the east and north east of Iran. The plant was collected in the middle of spring 1999, dried in the shade, ground and defatted by maceration using petroleum ether. Extraction was completed by applying ethanol (85%). Total extraction of Salvia leriifolia was divided into 4 different fractions using liquid-liquid extraction procedure applying 3 solvents (Dichloromethan, n- Hexan and Isobutanoul). The plants were tested by TLC for absence of histidine and-contamination with aflatoxin before proceeding with the extraction. The other steps were as follow: 1) Genotypes of three standard tester strains (TA98, TA100 and TA102 of Salmonella typhimurium ) were confirmed with study five important factors that were: histidine requirement, rfa mutation, uvrB mutation, presence of R factor and pAQ1 plasmid. 2) The MIC of fractions were detemined. 3) The antimutagenic effects of 4 different concentrations of each fraction (less than 0.1 MIC) were studied in absence and presence of S9. The sample, tester strains and mutagen specific for each tester strain were incoporated into the top agar of atnimutagenisity assay plates and incubated for 48 hrs. at 37 °C. The revertant colonies were counted and compared with control plates. Analysis of the results showed antimutagenic effects for aquatic and isobutanulic fractions.