در گونه هاي مختلف رت IGE و ارتباط آن با توليد RT-PCR اينترلوكين 4 به وسيله MRNA بررسي سطح بيان

Analysis of IL-4 mRNA level by RT-PCR and association with IgE production in different rat strains

Background Interleukin-4 (IL-4) is a key anti-inflammatory cytokine and plays an important role in the cytokine network and in the Thl/Th2 balance. It is generated by helper T cells, and possibly in the early adaptive immune response by mast cells and basophiles, and like all cytokine generally expressed at low levels with a short half-life of the mRNA. There are controversial evidences suggesting the role of IL-4 and IL-13 in the pathogenesis of allergy and asthma. The purpose of this research was to indicate the role of IL-4 gene expression in allergic and non-allergic rat models. We tested three different rat strains, "allergic" high IgE producing BN (Brown Norway) strain rats, "non-allergic" low IgE responder PVG strain rats and low IgE producing WISTAR strain rats, for IL-4 mRNA and IL-4 protein expression by a semi-quantitative RT-PCR and ELISA methods. The results showed that BN generated the highest amount of IL-4 and mRNA expression level. WISTAR strain and PVG strain showed lower amount of IL-4 expression in all experiments and can be looked at the lowest IL-4 mRNA producer. Our results demonstrated the hypothesis that higher expression of 1L-4 mRNA may be "allergic" and high IgE producing. Although we can not exclude the polymorphism of the IL-4 gene in the non-coding region and the role of other cytokine like IL-13 and TGF-J3 in the cytokine network.