تغييرات گليكوكونجو گيت ها در سلولهاي جزاير لانكرهانس از مرحله جنيني به نوزادي

Fetal to neonatal glycoconjugates changes of islet cells

Pancreatic cells arise from ventral and dorsal buds of the foregut endoderm during early morphogenic period of intrauterine life. It is believed that endocrine cells might be derived from the APUD system which have neuroepithelial origin. Little is known about the involvement of glycoconjugates in regulating islet cells during course of their embryonic development. In this study, lectin histochemical technique was performed on formalin or B 4 G-fixed paraffin embedded pancreatic tissue during late fetal and early postnatal periods in rats to identifyin situ pattern of carbohydrate residues, during pancreatic histogenesis. Both endocrine and exocrine pancreatic cell types, reacted differently with MPA for Galactose and SBA for GalNac terminal sugars. Based on these reactions, we were able to distinguish different cell types of islets of Langerhans during late fetal period. Interestingly that all islets reactions were disapeared after the first week of postnatal life. Luminal surface of exocrine acini reacted with both lectins and stayed the same during postnatal days. The potential significance of these observations and the role of glycoconjugates in islet cells differentiation are discussed.