AgNOR افتراق بين كارسينوم و هيپرپلازي تدولر پرومستات با استفاده از رنگ آميزي

Differentiation between prostatic carcinoma and nodular prostatic hyperplasia by AgNOR stainig

Background: Differentiation between prostatic carcinoma and hyperplasia may not be difficult in most cases, but sometime it is a problem even for expert pathologists. The method of AgNOR staining was applied for evaluating the usefulness of it in distinguishing between benign and malignant lesions. Methods: 50 cases of prostatic carcinoma and 50 cases of nodular prostatic hyperplasia were selected and stained by the AgNOR (argirophilic nucleolar organizer region) method. These included prostatectomy (open/T.U.R.) or needle biopsy specimens of the prostate. Prostatic cancers were classified and graded according to Gleasonʹs Histologic Grading System. Results: In prostatic hyperplasia the mean number of AgNOR count was 1.35 per cell and in prostatic carcinoma it was 3.77. Prostatic hyperplasia showed one or two well defined NORs, but in prostatic carcinoma there were many large irregular NORS. In prostatic hyperplasia the average percentage of cells exhibited three or more interphase AgNORs, which were 7.46% and in the prostatic carcinoma 72.28% . Conclusions: AgNOR stain is useful for differentiation between prostatic carcinoma and hyperplasia, but this stain may not be used as a reliable index for grading of prostatic adenocarcinoma, because overlapping was seen in some cases.