اثرات جنستين مهاركننده تيروزين كيناز، در التهاب حاد و مزمن در موش هاي ديابتي

The effects of genistein, a tyrosine kinase inhibitor, on acute and chronic inflammation in diabetic mice

The effects of tyrosine kinases on acute and chronic inflammation during diabetes are not fully determined. Therefore, the present study focuses on investigating the effects of genistein, a tyrosine kinase inhibitor, on acute and chronic inflammation in diabetic mice. The mice either received normal saline (control, 0.1 ml, i.p., n=144) or were injected with streptozotocin (diabetic, STZ, 200 mg kg i.p., n=144). By injecting carrageenan and implanting 2 cotton pellets (a week after the injection of saline or STZ) we induced acute and chronic inflammation. Before injecting carrageenan or 5 day after implantation, 9 mice from each group (control or diabetic) received genistein (10 mg kg^-1, i.p.), indomethacin (2 mg kg ^-1, i.p.) or L-NAME (0.1 mg kg i.p.). Paw edema and the weight of cotton pellets were significantly higher in diabetic mice. Pretreatment with either indomethacin or L-NAME significantly reduced the acute and chronic inflammation in the diabetic group. Genistein reduced chronic inflammation significantly (P<0.0001). These results suggest that activation of tyrosine kinases as well as prostaglandins and nitric oxide pathways are involved in the increased chronic inflammatory responses observed in the diabetic animals.