تعيين ميزان تجزيه پذيري علوفه با استفاده از فن توليد گاز و كيسه هاي نايلوني

Determination of Roughages Degradability through In vitro Gas Production and Nylon Bag Techniques

The nylon bag method and in vitro gas production techniques were used to describe the kinetics of fermentation based on the exponential model P = a + b (1-e^-et). Alfalfa hay (ALF), phragmites australis (PA) or wheat straw (WS) were offered twice a day (8:00 and 16:00) at about maintenance level to 3 ruminaly fistulated Sistani (Bas indicus) and 3 Holstein (Bas taurus) cattle in a completely randomized design of factorial arrangement. Dry matter (DM) and crude protein (CP) degradability and gas volume (ml 1200 mg DM) were measured after 0, 4, 8, 12, 24, 48, 72 and 96 hours of incubation. Taking into consideration the in vitro gas production as related to nylon bag results, there were no significant differences observed in DM and CP degradability of ALF, PA or WS incubated in the rumen of the two cattle breeds, the same pattern being observed for total cummulative in vitro gas production. When feed samples were incubated for 24 hours, gas volume (ml 1200 mg DM) of ALF (41.63) was higher than in PA (29.17) and in WS (26.02) samples (p<0.05). The same pattern was observed for DM and CP degradability of ALF (46.23%, 79.63%) , PA (37.67%, 58.42%) and WS (36.24%, 41.95%) respectively (p<0.05). The high correlation coefficient (r) of in vitro gas production with degradability coefficient for ALF, PA and WS provide validation as to the use of gas production test as an appropriate in vitro technique for evaluating these roughage feeds.