ارزيابي چندين رقيق كننده براي نگهداري اسپرم خروس هاي بومي فارس در دماي 5-4 و 24-19 درجه ي سانتيگراد

An Evaluation of Several Semen Diluents for Storage Of Fars Native Chicken Sperm at 4-5 and 19-24

An experiment was conducted to investigate the effectiveness of several diluents for storing, native chicken sperm at either 4-5 C .(Sexton; Van Wembeke; and Lake and Ravie diluents) or room temperature (19-24 C) (Chaudhuri and Lake; and Sexton diluents). Seminal charactristics (%live sperm, sperm motility and pH) were determined immediately upon dilution (Time 0) and at 6 and 24 h after storage. An aliquot (0.2 ml) of diluted semen (1:2 ratio) stored at 0, 6 and 24 h was inseminated in the afternoon. Each hen was inseminated once a week for 5 weeks while eggs being collected for determination ofʹ fertilizability and hatchability. Diluent type, storage time, and storage temperature significantly affected sperm motility. % live sperm, pH. 0 fertile eggs and o hatchability. ofʹ incubated eggs. Sperm motility, % live sperm,fertile eggs and ) hatchability were significantly higher for the semen stored at 4-5 C as compared with storage at room temperature. Storage time adversely affected the parameters under investigation. All diluents were suitable for immediate insemination after dilution (91 to 99% fertlity), and the lowest fertility (1.2%) was found in hens inseminated with Chaudhun and Lakes diluent stored for 24 h at room temperature. Fertility of diluted semen samples stored at 4-5 C was higher than that of samples stored at room temperature. Compared with other diluents. Sexton resulted in higher fertility levels for the sperms that were stored at 4-5 C" for either 6 It (77%) or 24 h (41%). Correlation coefficients of sperm motility (or % live sperm) with °,o fertile eggs and ʹ10 hatchability of incubated eggs were high (r=0.50 to 0.90) and significant (P<0.0001 ). The correlation coefficients of p11 of diluted semen with sperm motility. (r=0.65), °.o live sperm (0.71) and of fertility (r-0.67) were also significant (P<0.0001).