ارزيابي و معرفي ژنوتيپ هاي مقاوم به ويروس در سيب زميني

Identification and Introduction of Virus Resistat Genotypes in

Since one of the important potato diseases in our country is potato virus, PVX and PVY, so, identification and introduction of viruses resistat cultivars is of paramont importance. In this research, infection was artificially carried out using PVX and PVY viruses. In order to produce free virus genotypes, meristem culture was employed. Meristem explants that included 2 or 4 leaves ( 0.6 , 0.12 , 0.27 mm length) were cultured on liquid as well as solid germination media with different hormones of Kinetin, ABA , BAP, GA of different doses ( 0, 0.05 , 0.1 , 0.5 , 0.7 , I mg 11 ). Shoots for regeneration of complete plant were transferred into media with NAA , 2,4 - D, IBA,IAA (0, 0.05 , 0.1, 0.5, 0.7, 1 mg 11 ). Data analysis in meristem culture (factorial, complete random design) indicated a significant difference between genotypes, hormones, media and meristem length. Sante 0.6 mm meristem x agar media x BAP ( 0.7 mg/l ) was introduced as the best shoot inducing combination. NAA (0.7 mg/l) among root inducing media induced the highest regeneration (76%). ELlSA test was done and after recognition of healthy seedlings, artificial infection by PVX ( mecanical ) and PVY ( vector ) in green house as well as in vitro was done and again ELlSA test was carried out after 2 weeks. Sante 80397007, 396278, 396140, exhibited resistance to PVY , PVX with Mondial showing resistance only to PVX. In vitro results were the same as those in green house.