به ژنوتيپ هاي مختلف گندمهاي هگزا پيلوييد و تتراپلوييد )Triticum aestivuml.( مطالعه انتقال پذيري ماركرهاي ميكروساتلايت گندم هگزاپلوييد

Transferability of Hexaploid Wheat (Triticum aestivum)Microsatellite Markers to Hexaploid and Tetraploid Wheat Species

Wheat is one of the worldʹs most important crop plants and understanding its genetic and genome organization using molecular markers is of great value for genetic and plant breeding purposes. In this study, we examined transferability of 96 microsatellites markers from hexaploid wheat to 5 hexaploid and tetraploid wheat cultivars (T. aestivum L cv. Mironovska 808, T. aestivum L cv. Chinese spring, T. aestivum L. cv. T. durum Des£ cv. Langdon, Triticum turgidum ssp. dicoccoides; and T. turgidum ssp. dicoccum). Results showed that, among the 96 primer pairs, 95 (98.96%) amplified microsatellite loci while only one microsatellite marker (1.04%) failed to amplify any products in the five cultivars. Among the 5 cultivars, informative polymorphic banding patterns were observed at 73 (53.5%) out of 96 microsatellites loci. Twenty two (22.92%) primer pairs produced amplification products on some cultivars, so that, their highest transferability was 80% and the lowest 20%. Also in this study, extensive polymorphism was observed as detected by differences in the size and number of the amplification products. The results indicated that, most of the microsatellite markers derived from hexaploid wheat (98.96%) showed quite good transferability towards the hexaploid and tetraploid species and it might be possible to facilitate more widespread use of microsatellites in agriculturally important plants. Also this result can lead to the provision of an additional source of markers for gene mapping, linkage analysis and genome evolution studies in hexaploid and tetraploid wheat species.