بررسي كشت بافت و اثر كشت همجواري ريزنمونه ها در كلزا

In vitro Culture and Effects of Explants coculturing in Canoltr(Brossica napus L.)

In order to investigate callus induction and regeneration in canola, hypocotvledonary explants from 6-day seedlings were cultured in MS medium complemented with 2mg Iif BAP and 1 mg lit^-1 NAA..Callus initiation_ was earlier in Synl with the most callus induction occurring in PF7045/91. Calli in Okapi and Culvert were greater than in other genotypes. Subculturing of callus pieces, decreased callus inductions as well as organ genesis dramatically in all genotypes. After subculturing of calli in two regeneration media with different growth regulators, shoot regeneration in RegentxCobra was more than in others. ANOVA for callus volume showed that media and genotypes were significantly different, with embryogenesis being significant only for genotypes. In all these procedures SLM.046 didnʹt respond to hypocotyledon in vitro culture. In coculturing of different explants- of Colvert genotype in modified MS (excluding Pyridoxine - HC1 and Nicotinic Acid, and including 0.4 mg Iif ^-1Thiamin - HCI, 2 mg lif^-1 BAP and 0.2 mg lift NAA) cotyledon - hypocotyl. cultures had the best callus induction response and in all of the obtained calli, embryogenesis occurred. But shoot regeneration was observed only in cotyledon culture. In this research, root cultured; with other Explants, seriously influenced callus induction and their differentiation. It seems that these effects are related to special substances in root explants, the identification of which would lead to epigenetic variation in callus cultures.