با آگروباكتريوم ريزوژنز براي مطالعه بيان ژن در ريشه هاي مويين BETAVULGORIS L تراريختي چغندرقند

Agrobacterium rhizogenes Mediated Transformation of Sugar Beet ( Beta vulgaris L.) for Studying Gene Expression in Hairy Roots

In this research, sugarbeet transformed hairy root was produced by Agrobacterium rhizogenes mediated transformation. Effect of a promoter (AT) derived from Arabidopsis thaliana was compared with CaMV35S promoter for expression of one reporter gene. AT promoter was very similar to Hs11 m. promoter that is responsible for nematode resistance in sugar beet. As a negative control, hairy roots were induced to Agrobacterium rhizogenes without binary vector. Then, hairy roots were analyzed by PCR and Southern hybridization. Positive hairy roots were selected and inoculated with nematode larvae. After 10 days, hairy roots were stained by GUS assay. The results indicated that hairy roots harboring AT promoter, have similar staining pattern to hairy roots harboring CaMV35S promoter. Therefore, it seems AT promoter although having much similarity to Hsl^pro-1 inducing promoter, isnʹt an inducing one, so that GUS stain spots could be observed in all parts of hairy root specially in meristemic zone and far from nematode cyst site. In conclusion, AT promoter has been found to have acted as a constitutive promoter.