با اندام زايي مستقيم در قطعات رولپه )citrus siensis L.Osbeck( افزايش درون شيشه اي پرتقال

IN VITRO PROPAGATION OF SWEET ORANGE (Citrus sinensis L. Osbeck) BY DIRECT ORGANOGENESIS IN EPICOTYL SEGMENTS

Experiments were conducted to study in vitro propagation of sweet orange using 5mm long epicotyl explants. Explants were excised from in vitro seedlings and cultured on a basic MS medium supplemented with various concentrations of benzyl adenine (BA) and naphthalene acetic acid (NAA). After 60 days, the regeneration of adventitious shoots was recorded. The maximum percentage of regeneration and maximum number of adventitious shoots were obtained in BA concentrations of I and 2 mg 1-1 Increasing BA concentration, decreased the production of adventitious shoots. Concentrations of 0.5 and I mg 1-1 NAA had not a significant effect on regeneration and number of adventitious shoots. However in low concentrations of BA, NAA increased the length of adventitious shoots, and maximum length of adventitious shoots was obtained on media containig I mg 1-1 BA and I mg 1-1NAA. For rooting, basal medium with half strength of MS salts plus 3% sucrose, 7.5 g 1-1 agar and NAA or indolebutryic acid (IBA) was used. A five-second dip of basal ends of shoots in 1000 mg 1-1 NAA or IBA solutions and transferring to half strength MS salts medium were also evaluated for rooting. The maximum percentage of rooting was obtained on IBA-containing media and NAA did not affect rooting. Additing NAA to medium resulted in callus formation in basal cut ends of shoots. The maximum percentage of rooting and root number and length were obtained in 10 mg 1-1IBA. Plantlets were transferred to pots containing a mixture of 50% soil and 50% sand, and were irrigated by one-eighth strength MS salt solution for 10 days and then gradually acclimized to natural environment.