3[ H] سنتز اسيدآمينه لوسين نشاندار با تريتيوم

Synthesis of the DL-[3H] Leucine

in life, science and chemistry. The preparation of labeled compounds, with carbon-14 and tritium-3 arc probably more extensively and variously used in compare with any other isotopes. These isotopes emit only beta-particle. In this paper the synthesis of DL [3H] Leucine which was prepared for the first time in Iran is described. This compound is used for diagnosis of talasemi disease. The synthetic pathway is achieved by using the condensation of methyl ally1 chloride with diethyl acetamido malonate in the presence of sodium ethoxid in ethanol .In the next step of the synthesis, the latter compound was hydrogenated with 3H2(g) over the Adams catalyst in chloroform, which was produced ethyl 2-acetamido-2- carbetoxy -4-methyl pentanoate with a yield of 100%. in final step, the resulted product was converted directly to [4,5-3H] Leucine with the specific activity 5 Ci/mmol, by refluxing with the hydrobromic acidof48%. Labeled organic compounds have been widely applied to solve the research problems.