تاثير نقش شوري ناشي از كلرورسديم بر پروفيل الكتروفورزي پروتيين ها و آنزيم هاي گندم

Effect of Salt Stress (NaCl) on Electrophoretic Pattern of Wheat Proteins and Enzymes

The present investigation was carried out to study soluble proteins and antioxidant enzymes changes in wheat affected by salt stress. Six Iranian wheat cultivars and a line, differing in salt tolerance, were treated in hydroculture media with four different concentrations of NaCl (0, 50, 100 and 150 mM). The young leaves and roots were harvested. The soluble leaf proteins and root enzymes were treated by Tris-HCl buffers for electrophoresis analysis. There was no clear evidence for a leaf soluble protein, corresponding to salt tolerance, nevertheless, solely the density of 32 and 56 kilodalton polypeptide bands in Mahdavi and Chenab cultivars showed some increase. However the activity of peroxidase and catalase which were increased in roots, suggesting that the salt stress induced the activity of antioxidant defence system, due to the presence of active oxygen radicals.