بررسي اثرات متقابل فيتوهماگلوتينين )PHA( با سيتوكينين ها بر روي كال زايي و ارگانوژنزنسج گياهان توتون

The effect of phytohemagglutinin (PHA) and cytokinines interactions on the callus production and organogenesis of Nicotiana plant tissue

In this research the interaction of phytohemagglutinin (PHA) with cytokinines Banzil-Adenine, Forfiril-Amin and Adenine on the callus production and organogenesis of Nicotiana plant tissue was investigated. For this purpose MS basal medium and different concentrations of cytokinines and PHA were applied. Two levels (2 and 3 ppm) of PHA and two levels (0.1 and 1 ppm) of cytokinines were used. The experiment was conducted as a completely randomized design with three replication. Explants from fifth leaf lamina of Nicotiana tabacum were selected and cultured on the culture medium. Subsequently, explants transfered to 26±2 degree of centigrade under 16 h. daylight with 1500 lux intensity. The fresh and dry weight explants after fourty days were recorded. Because of heteroscedasticity within treatments in the experiment, the treatments were divided into two groups (groups one and two) and analysis of variance was performed for each group separately. Statistical analysis showed significant differences among the treatments in the two groups for fresh weight, but for dry weight the differences were observed among the first group. Comparision of treatment means by Duncanʹs - multiple range test (p = %5) indicated that the treatment of (BA 1ppmx PAH 2ppm) and treatment of (BA IppmxPHA 3ppm) for fresh weight and treatment of (FA IppmxPHA 3ppm), (BA IppmxPHA 2ppm), (BA IppmxPHA 3ppm) and (Fd IppmxPHA 2ppm) for dry weight were superior than other treatments. So the treatments with BA 1ppm or FA 1ppm and 2 or 3ppm of PHA showed fresh and dry weight more than other treatments, and produce seedling from steam and root.