استفاده از روش RAPD جهت بررسي پلي مورفيسم DNA در سويه هاي جدا شده قارچ فوزاريوم اگزيسپوروم

Using Random Amplified Polymorphism DNA (RAPD) Technique for DNA Polymorphism in Isolated Strains of Fusarium oxysporum

Background and Objectives: Applying DNA based markers specially PCR dependent techniques including Random Amplified Polymorphism DNA (RAPD) is one of the powerful tools for studying genetic variations in living organisms. The aim of this study was to identify genetic variations among different isolated Fusarium Oxysporum species by using molecular markers ofʹ RAPD. Materials and Methods: 15 strains of Fusarium oxysporum were used in this research. Using modified Dellaporta method performed DNA extraction. After PCR amplification, to study the pattern of DNA polymorphism, the PCR products were ran on 2% agarose gel electrophoresis along with using DNA size marker. For statistic analysis of RAPD products applying SPSS software used Ward procedure. Results: The obtained results by using 12 primers, all strains were divided into three groups. These results showed that the west provinces of the country have similar pattern of DNA polymorphism whereas all strains with having low genetic diversity were put in the same group. However, the isolated strains of east-west provinces of Iran because of having different patterns of polymorphism were located in three different groups. Conclusion: Comparison of RAPD pattern, in strains, based on geographical area showed an association between RAPD patterns of strains obtained from west parts of Iran with geographical origin. On the other hand, RAPD could be able to recognize the difference between isolated strains of this area with other locations.